The effect of erythropoietin on autologous stem cell-mediated bone regeneration

Ashwin M. Nair, Yi Ting Tsai, Krishna M. Shah, Jinhui Shen, Hong Weng, Jun Zhou, Xiankai Sun, Ramesh Saxena, Joseph Borrelli, Liping Tang

Research output: Contribution to journalArticle

37 Scopus citations

Abstract

Mesenchymal stem cells (MSCs) although used for bone tissue engineering are limited by the requirement of isolation and culture prior to transplantation. Our recent studies have shown that biomaterial implants can be engineered to facilitate the recruitment of MSCs. In this study, we explore the ability of these implants to direct the recruitment and the differentiation of MSCs in the setting of a bone defect. We initially determined that both stromal derived factor-1alpha (SDF-1α) and erythropoietin (Epo) prompted different degrees of MSC recruitment. Additionally, we found that Epo and bone morphogenetic protein-2 (BMP-2), but not SDF-1α, triggered the osteogenic differentiation of MSCs invitro. We then investigated the possibility of directing autologous MSC-mediated bone regeneration using a murine calvaria model. Consistent with our invitro observations, Epo-releasing scaffolds were found to be more potent in bridging the defect than BMP-2 loaded scaffolds, as determined by computed tomography (CT) scanning, fluorescent imaging and histological analyses. These results demonstrate the tremendous potential, directing the recruitment and differentiation of autologous MSCs has in the field of tissue regeneration.

Original languageEnglish (US)
Pages (from-to)7364-7371
Number of pages8
JournalBiomaterials
Volume34
Issue number30
DOIs
StatePublished - Oct 2013

Keywords

  • Autologous stem cells
  • Bone morphogenetic protein-2
  • Bone tissue engineering
  • Erythropoietin
  • Scaffold
  • Stromal derived factor-1α

ASJC Scopus subject areas

  • Bioengineering
  • Ceramics and Composites
  • Biophysics
  • Biomaterials
  • Mechanics of Materials

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