The expression of a functional cDNA encoding the chicken cytochrome P-450(arom) (aromatase) that catalyzes the formation of estrogen from androgen

M. J. McPhaul, J. F. Noble, E. R. Simpson, C. R. Mendelson, J. D. Wilson

Research output: Contribution to journalArticle

150 Scopus citations

Abstract

A complementary DNA (cDNA) copy of the aromatase P-450 has been isolated from a chicken ovary library using as probe a partial cDNA believed to encode the human placental aromatase. The predicted amino acid sequence of the chicken aromatase cDNA possesses regions of homology to that of its human counterpart, but only limited homology to other cytochrome P-450 enzymes. The introduction of the cDNA clone into COS-1 cells results in the production of high levels of aromatase activity. The chicken enzyme is targeted to the appropriate subcellular fraction in the transfected COS cells, and the apparent K(m) of the chicken aromatase activity, measured in microsomes prepared from the transfected cells, is similar to that of the enzyme prepared from the chicken ovary microsomes. These findings establish that the cDNA clone encodes chicken ovarian aromatase and demonstrate that this protein can catalyze the three successive oxidation reactions necessary to form estrogen from androgen.

Original languageEnglish (US)
Pages (from-to)16358-16363
Number of pages6
JournalJournal of Biological Chemistry
Volume263
Issue number31
StatePublished - Jan 1 1988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

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