The frequency of homologous recombination in human ALT cells

Oliver E. Bechter, Jerry W. Shay, Woodring E. Wright

Research output: Contribution to journalReview articlepeer-review

Abstract

Instead of telomerase, some immortal cells use the alternative lengthening of telomeres pathway (ALT) to maintain their telomeres. There is good evidence that homologous recombination contributes to the ALT mechanism. Using an inducible GFP reporter system to measure the frequency of homologous recombination, we asked whether or not ALT cells exhibited a general change of the recombination machinery. Our results show that the frequency of homologous recombination for non-telomeric sequences in ALT cells is identical to that in telomerase positive cells, irrespective of whether the reporter was present at an intra-chromosomal location or next to a telomeric sequence. We conclude that the underlying recombination defect in ALT cells is restricted to telomeric sequences.

Original languageEnglish (US)
Pages (from-to)545-547
Number of pages3
JournalCell Cycle
Volume3
Issue number5
DOIs
StatePublished - May 2004

Keywords

  • ALT
  • Cancer
  • Double strand break
  • Homologous recombination
  • Immortalization
  • Telomerase
  • Telomere length

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

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