The full-of-bacteria gene is required for phagosome maturation during immune defense in Drosophila

Mohammed Ali Akbar, Charles Tracy, Walter H A Kahr, Helmut Krämer

Research output: Contribution to journalArticle

46 Citations (Scopus)

Abstract

Arthrogryposis, renal dysfunction, and cholestasis (ARC) syndrome is a fatal recessive disorder caused by mutations in the VPS33B or VPS16B genes. Both encode homologues of the Vps33p and Vps16p subunits of the HOPS complex necessary for fusions of vacuoles in yeast. Here, we describe a mutation in the full-of-bacteria (fob) gene, which encodes Drosophila Vps16B. Flies null for fob are homozygous viable and fertile. They exhibit, however, a defect in their immune defense that renders them hypersensitive to infections with nonpathogenic bacteria. fob hemocytes (fly macrophages) engulf bacteria but fail to digest them. Phagosomes undergo early steps of maturation and transition to a Rab7-positive stage, but do not mature to fully acidified phagolysosomes. This reflects a specific requirement of fob in the fusion of phagosomes with late endosomes/lysosomes. In contrast, cargo of autophagosomes as well as endosomes exhibit normal lysosomal delivery in fob cells. These findings suggest that defects in phagosome maturation may contribute to symptoms of ARC patients including recurring infections.

Original languageEnglish (US)
Pages (from-to)383-390
Number of pages8
JournalJournal of Cell Biology
Volume192
Issue number3
DOIs
StatePublished - Feb 7 2011

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Phagosomes
Drosophila
Bacteria
Genes
Endosomes
Diptera
Hemocytes
Mutation
Vacuoles
Lysosomes
Infection
Yeasts
Macrophages

ASJC Scopus subject areas

  • Cell Biology

Cite this

The full-of-bacteria gene is required for phagosome maturation during immune defense in Drosophila. / Akbar, Mohammed Ali; Tracy, Charles; Kahr, Walter H A; Krämer, Helmut.

In: Journal of Cell Biology, Vol. 192, No. 3, 07.02.2011, p. 383-390.

Research output: Contribution to journalArticle

Akbar, Mohammed Ali ; Tracy, Charles ; Kahr, Walter H A ; Krämer, Helmut. / The full-of-bacteria gene is required for phagosome maturation during immune defense in Drosophila. In: Journal of Cell Biology. 2011 ; Vol. 192, No. 3. pp. 383-390.
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