TY - JOUR
T1 - The G protein G13 mediates inhibition of voltage-dependent calcium current by bradykinin
AU - Wilk-Blaszczak, M. A.
AU - Singer, W. D.
AU - Gutowski, S.
AU - Sternweis, P. C.
AU - Belardetti, F.
N1 - Funding Information:
Hepler for rG,,, rG,&, and anti-GT4, IgG, and to K. Edwards for expert and patient administrative assistance. This work was supported by NIH grants to P. S. (ROI GM31954) and F. B. (ROI GM47721), a NIH Fellowship to W. S. (F32 GM15359), and a Klingenstein Award to F. B. F. B. is an Established Investigator of the American Heart Association.
PY - 1994/11
Y1 - 1994/11
N2 - In neuroblastoma-glioma hybrid cells, bradykinin has dual modulatory effects on ion channels: it activates a K+ current as well as inhibits the voltage-dependent Ca2+ current (ICa,V). Both of these actions are mediated by pertussis toxin-insensitive G proteins. Antibodies raised against the homologous Gq and G11 proteins suppress only the activation of the K+ current; this suggested that at least two distinct G protein pathways transduce diverse effects of this transmitter. Here, we show that the inhibition of ICa,V by bradykinin is suppressed selectively by intracellular application of antibodies specific for G13. This novel G protein may play a general role in the inhibition of ICa,V by pathways resistant to pertussis toxin.
AB - In neuroblastoma-glioma hybrid cells, bradykinin has dual modulatory effects on ion channels: it activates a K+ current as well as inhibits the voltage-dependent Ca2+ current (ICa,V). Both of these actions are mediated by pertussis toxin-insensitive G proteins. Antibodies raised against the homologous Gq and G11 proteins suppress only the activation of the K+ current; this suggested that at least two distinct G protein pathways transduce diverse effects of this transmitter. Here, we show that the inhibition of ICa,V by bradykinin is suppressed selectively by intracellular application of antibodies specific for G13. This novel G protein may play a general role in the inhibition of ICa,V by pathways resistant to pertussis toxin.
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U2 - 10.1016/0896-6273(94)90059-0
DO - 10.1016/0896-6273(94)90059-0
M3 - Article
C2 - 7946358
AN - SCOPUS:0028170587
SN - 0896-6273
VL - 13
SP - 1215
EP - 1224
JO - Neuron
JF - Neuron
IS - 5
ER -