The liver has been shown to contain a facilitated diffusion glucose transporter with high K(m) for glucose that is structurally distinct from the low K(m) glucose transporters found in most other tissues. We find that 3-O-methyl gluose is greater than 90% equilibrated across dispersed islet cells within 60 s, consistent with a facilitated diffusion transport mechanism. L-Glucose uptake was minimal throughout the time course, indicating stereospecificity. Measurement of glucose transport over a range of 3-O-methyl glucose concentrations from 0.05 to 60 mM revealed the presence of a component of glucose transport with an apparent K(m) of 17 mM, a value essentially identical to that previously reported for liver. Interestingly, a second component of glucose transport was also observed with an apparent K(m) of 1.4 mM, as has been reported for other tissues such as erythrocytes that are known to contain the 'HepG2' or 'erythroid/brain' type glucose transporter. Further evidence for the existence of two transport components is provided by the observation that a low concentration of cytochalasin B (0.4 μM) completely inhibits the low K(m) transport activity but has no effect on the high K(m) transporter. The kinetic similarity of high K(m) glucose transport in liver and islets is readily understood in light of our structural analysis. Sequence analysis of cDNA clones indicates that the liver and islet glucose transporters have identical sequences and, thus, are the products of the same gene.
|Original language||English (US)|
|Number of pages||4|
|Journal||Journal of Biological Chemistry|
|State||Published - May 16 1990|
ASJC Scopus subject areas
- Molecular Biology
- Cell Biology