The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides

Hua Lu, Adwait Amod Sathe, Chao Xing, Mark A Lehrman

Research output: Contribution to journalArticle

Abstract

Discovered 40 years ago, the Lec5 glycosylation mutant cell line has a complex recessive genotype and is characterized by accumulation of lipid-linked oligosaccharide assembly intermediates, reduced conversion of polyprenols to dolichols, and an unusual phenotypic dependence upon cell culture conditions such as temperature, plating density and medium quality. The heritable defect in Lec5 is unknown. Here we demonstrate an unexpected epigenetic basis for Lec5, with a surprising linkage to increased expression of homeobox genes, which in turn is associated with increased transcription of cholesterol biosynthesis genes. These results suggest testable hypotheses for the biochemical abnormalities of the Lec5 mutant.

Original languageEnglish (US)
Pages (from-to)106-109
Number of pages4
JournalGlycobiology
Volume29
Issue number2
DOIs
StatePublished - Feb 1 2019

Fingerprint

Dolichol
Glycosylation
Homeobox Genes
Epigenomics
Cell Culture Techniques
Genes
Cholesterol
Genotype
Cell Line
Temperature
Biosynthesis
Transcription
Cell culture
Plating
Cells
Defects
lipid-linked oligosaccharides

ASJC Scopus subject areas

  • Biochemistry

Cite this

The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides. / Lu, Hua; Sathe, Adwait Amod; Xing, Chao; Lehrman, Mark A.

In: Glycobiology, Vol. 29, No. 2, 01.02.2019, p. 106-109.

Research output: Contribution to journalArticle

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