The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides

Hua Lu, Adwait Amod Sathe, Chao Xing, Mark A. Lehrman

Research output: Contribution to journalArticle

Abstract

Discovered 40 years ago, the Lec5 glycosylation mutant cell line has a complex recessive genotype and is characterized by accumulation of lipid-linked oligosaccharide assembly intermediates, reduced conversion of polyprenols to dolichols, and an unusual phenotypic dependence upon cell culture conditions such as temperature, plating density and medium quality. The heritable defect in Lec5 is unknown. Here we demonstrate an unexpected epigenetic basis for Lec5, with a surprising linkage to increased expression of homeobox genes, which in turn is associated with increased transcription of cholesterol biosynthesis genes. These results suggest testable hypotheses for the biochemical abnormalities of the Lec5 mutant.

Original languageEnglish (US)
Pages (from-to)106-109
Number of pages4
JournalGlycobiology
Volume29
Issue number2
DOIs
StatePublished - Nov 2 2018

Keywords

  • cholesterol
  • dolichol
  • homeobox
  • lipid-linked oligosaccharide
  • polyprenol

ASJC Scopus subject areas

  • Biochemistry

Fingerprint Dive into the research topics of 'The Lec5 glycosylation mutant links homeobox genes with cholesterol and lipid-linked oligosaccharides'. Together they form a unique fingerprint.

  • Cite this