The Maintenance of Telomere Length in CD28+ T Cells during T Lymphocyte Stimulation

Ejun Huang, Enzo Tedone, Ryan O'Hara, Crystal Cornelius, Tsung Po Lai, Andrew Ludlow, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

8 Citations (Scopus)

Abstract

Telomerase activity is not readily detected in resting human T lymphocytes, however upon antigen presentation, telomerase is transiently upregulated. Presently, it is not known if telomerase activation is necessary for the proliferation of T cells or for the maintenance of telomere lengths. In this study, we found that telomerase activation is not required for the short- term proliferation of T cells and that telomeres progressively shorten in a heterogeneous population of T cells, even if telomerase is detected. By measuring telomerase activity at the single-cell level using quantitative ddPCR techniques (ddTRAP) and by monitoring changes in the shortest telomeres with more sensitive telomere length measurement assays, we show that only a subset of CD28+ T-cells have robust telomerase activity upon stimulation and are capable of maintaining their telomere lengths during induced proliferation. The study of this T-cell subset may lead to a better understanding on how telomerase is regulated and functions in immune cells.

Original languageEnglish (US)
Article number6785
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

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Telomere Homeostasis
Telomerase
Lymphocyte Activation
T-Lymphocytes
Telomere
T-Lymphocyte Subsets
Telomere Shortening
Antigen Presentation

ASJC Scopus subject areas

  • General

Cite this

The Maintenance of Telomere Length in CD28+ T Cells during T Lymphocyte Stimulation. / Huang, Ejun; Tedone, Enzo; O'Hara, Ryan; Cornelius, Crystal; Lai, Tsung Po; Ludlow, Andrew; Wright, Woodring E.; Shay, Jerry W.

In: Scientific Reports, Vol. 7, No. 1, 6785, 01.12.2017.

Research output: Contribution to journalArticle

Huang, Ejun ; Tedone, Enzo ; O'Hara, Ryan ; Cornelius, Crystal ; Lai, Tsung Po ; Ludlow, Andrew ; Wright, Woodring E. ; Shay, Jerry W. / The Maintenance of Telomere Length in CD28+ T Cells during T Lymphocyte Stimulation. In: Scientific Reports. 2017 ; Vol. 7, No. 1.
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