The Maintenance of Telomere Length in CD28+ T Cells during T Lymphocyte Stimulation

Ejun Huang, Enzo Tedone, Ryan O'Hara, Crystal Cornelius, Tsung Po Lai, Andrew Ludlow, Woodring E. Wright, Jerry W. Shay

Research output: Contribution to journalArticle

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Abstract

Telomerase activity is not readily detected in resting human T lymphocytes, however upon antigen presentation, telomerase is transiently upregulated. Presently, it is not known if telomerase activation is necessary for the proliferation of T cells or for the maintenance of telomere lengths. In this study, we found that telomerase activation is not required for the short- term proliferation of T cells and that telomeres progressively shorten in a heterogeneous population of T cells, even if telomerase is detected. By measuring telomerase activity at the single-cell level using quantitative ddPCR techniques (ddTRAP) and by monitoring changes in the shortest telomeres with more sensitive telomere length measurement assays, we show that only a subset of CD28+ T-cells have robust telomerase activity upon stimulation and are capable of maintaining their telomere lengths during induced proliferation. The study of this T-cell subset may lead to a better understanding on how telomerase is regulated and functions in immune cells.

Original languageEnglish (US)
Article number6785
JournalScientific Reports
Volume7
Issue number1
DOIs
StatePublished - Dec 1 2017

ASJC Scopus subject areas

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    Huang, E., Tedone, E., O'Hara, R., Cornelius, C., Lai, T. P., Ludlow, A., Wright, W. E., & Shay, J. W. (2017). The Maintenance of Telomere Length in CD28+ T Cells during T Lymphocyte Stimulation. Scientific Reports, 7(1), [6785]. https://doi.org/10.1038/s41598-017-05174-7