The major immunoreactive α-melanocyte-stimulating hormone (αMSH)-like substance found in human fetal pituitary tissue is not αMSH but may be desacetyl αMSH (adrenocorticotropin1-13NH2)

F. J H Tilders, C. R. Parker, A. Barnea, J. C. Porter

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Abstract

Pituitary glands were obtained from human abortuses during the second half of gestation. Acid extracts were made from the anterior and neurointermediate lobes, and αMSH immunonoreactivity (αMSH(i)) was quantified by RIA. αMSH(i) was found in both lobes of the pituitary gland, with 20-80% of the total pituitary αMSH(i) being present in extracts of the anterior lobe. Anterior and neurointermediate lobe extracts subjected to gel filtration on Sephadex G-50 revealed one peak of αMSH(i) having an elution profile identical to those of αMSH and desacetyl αMSH (ACTH1-13NH2). To characterize further the αMSH(i), extracts were subjected to high pressure liquid chromatography. No αMSH could be identified in extracts of the anterior lobe, and most of the αMSH(i) had an elution profile identical to that of desacetyl αMSH. Although small amounts of αMSH might be present in the neurointermediate lobe, most of the αMSH(i) in this lobe coeluted with desacetyl αMSH. Since αMSH was not converted to desacetyl αMSH during the extration and chromatographic procedures, we hypothesize that the predominant form of αMSH-like material in the human fetal pituitary gland may be desacetyl αMSH.

Original languageEnglish (US)
Pages (from-to)319-323
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume52
Issue number2
StatePublished - 1981

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Melanocyte-Stimulating Hormones
Fetus
Tissue
Pituitary Gland
High pressure liquid chromatography
Pituitary Hormones

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

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title = "The major immunoreactive α-melanocyte-stimulating hormone (αMSH)-like substance found in human fetal pituitary tissue is not αMSH but may be desacetyl αMSH (adrenocorticotropin1-13NH2)",
abstract = "Pituitary glands were obtained from human abortuses during the second half of gestation. Acid extracts were made from the anterior and neurointermediate lobes, and αMSH immunonoreactivity (αMSH(i)) was quantified by RIA. αMSH(i) was found in both lobes of the pituitary gland, with 20-80{\%} of the total pituitary αMSH(i) being present in extracts of the anterior lobe. Anterior and neurointermediate lobe extracts subjected to gel filtration on Sephadex G-50 revealed one peak of αMSH(i) having an elution profile identical to those of αMSH and desacetyl αMSH (ACTH1-13NH2). To characterize further the αMSH(i), extracts were subjected to high pressure liquid chromatography. No αMSH could be identified in extracts of the anterior lobe, and most of the αMSH(i) had an elution profile identical to that of desacetyl αMSH. Although small amounts of αMSH might be present in the neurointermediate lobe, most of the αMSH(i) in this lobe coeluted with desacetyl αMSH. Since αMSH was not converted to desacetyl αMSH during the extration and chromatographic procedures, we hypothesize that the predominant form of αMSH-like material in the human fetal pituitary gland may be desacetyl αMSH.",
author = "Tilders, {F. J H} and Parker, {C. R.} and A. Barnea and Porter, {J. C.}",
year = "1981",
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T1 - The major immunoreactive α-melanocyte-stimulating hormone (αMSH)-like substance found in human fetal pituitary tissue is not αMSH but may be desacetyl αMSH (adrenocorticotropin1-13NH2)

AU - Tilders, F. J H

AU - Parker, C. R.

AU - Barnea, A.

AU - Porter, J. C.

PY - 1981

Y1 - 1981

N2 - Pituitary glands were obtained from human abortuses during the second half of gestation. Acid extracts were made from the anterior and neurointermediate lobes, and αMSH immunonoreactivity (αMSH(i)) was quantified by RIA. αMSH(i) was found in both lobes of the pituitary gland, with 20-80% of the total pituitary αMSH(i) being present in extracts of the anterior lobe. Anterior and neurointermediate lobe extracts subjected to gel filtration on Sephadex G-50 revealed one peak of αMSH(i) having an elution profile identical to those of αMSH and desacetyl αMSH (ACTH1-13NH2). To characterize further the αMSH(i), extracts were subjected to high pressure liquid chromatography. No αMSH could be identified in extracts of the anterior lobe, and most of the αMSH(i) had an elution profile identical to that of desacetyl αMSH. Although small amounts of αMSH might be present in the neurointermediate lobe, most of the αMSH(i) in this lobe coeluted with desacetyl αMSH. Since αMSH was not converted to desacetyl αMSH during the extration and chromatographic procedures, we hypothesize that the predominant form of αMSH-like material in the human fetal pituitary gland may be desacetyl αMSH.

AB - Pituitary glands were obtained from human abortuses during the second half of gestation. Acid extracts were made from the anterior and neurointermediate lobes, and αMSH immunonoreactivity (αMSH(i)) was quantified by RIA. αMSH(i) was found in both lobes of the pituitary gland, with 20-80% of the total pituitary αMSH(i) being present in extracts of the anterior lobe. Anterior and neurointermediate lobe extracts subjected to gel filtration on Sephadex G-50 revealed one peak of αMSH(i) having an elution profile identical to those of αMSH and desacetyl αMSH (ACTH1-13NH2). To characterize further the αMSH(i), extracts were subjected to high pressure liquid chromatography. No αMSH could be identified in extracts of the anterior lobe, and most of the αMSH(i) had an elution profile identical to that of desacetyl αMSH. Although small amounts of αMSH might be present in the neurointermediate lobe, most of the αMSH(i) in this lobe coeluted with desacetyl αMSH. Since αMSH was not converted to desacetyl αMSH during the extration and chromatographic procedures, we hypothesize that the predominant form of αMSH-like material in the human fetal pituitary gland may be desacetyl αMSH.

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