The mouse Ifi200 gene cluster: Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster

Stéphane Deschamps, Jeffrey Meyer, Gouri Chatterjee, Hong Wang, Peter Lengyel, Bruce A. Roe

Research output: Contribution to journalArticle

31 Citations (Scopus)

Abstract

The interferon-activatable Ifi200 gene cluster is located on mouse Chromosome 1q21-q23. We report here our analysis of two genomic regions encoding at least 10 closely related 200 family genes (Ifi201, Ifi202a, Ifi202b, Ifi202c, Ifi203a, Ifi203b, Ifi203c, Ifi203′, Ifi204, and Ifi204′) in 129/SvJ mice. Through a BAC-based sequencing approach, the exact structure and organization of these highly similar Ifi200 genes were obtained. A high degree of conservation (99% identity) was observed between Ifi202a and b and between Ifi203a and b. The presence of an additional transcribed region in intron 4 of Ifi203a and b suggests the possibility of alternative splicing, and a spliced variant of the Ifi204′ mRNA exhibits 91% sequence identity with a related but unmapped D3 mRNA. Comparative analysis of the mouse and human clusters indicates an absence of significant sequence conservation in noncoding sequences, suggesting that the 200 family emerged prior to human-mouse speciation and subsequently diverged after gene duplication.

Original languageEnglish (US)
Pages (from-to)34-46
Number of pages13
JournalGenomics
Volume82
Issue number1
DOIs
StatePublished - Jul 1 2003

Fingerprint

Multigene Family
Sequence Analysis
129 Strain Mouse
Messenger RNA
Gene Duplication
Alternative Splicing
Introns
Interferons
Genes
Chromosomes

Keywords

  • Cluster
  • Comparison
  • Evolution
  • Interferon
  • Sequence

ASJC Scopus subject areas

  • Genetics

Cite this

The mouse Ifi200 gene cluster : Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster. / Deschamps, Stéphane; Meyer, Jeffrey; Chatterjee, Gouri; Wang, Hong; Lengyel, Peter; Roe, Bruce A.

In: Genomics, Vol. 82, No. 1, 01.07.2003, p. 34-46.

Research output: Contribution to journalArticle

Deschamps, S, Meyer, J, Chatterjee, G, Wang, H, Lengyel, P & Roe, BA 2003, 'The mouse Ifi200 gene cluster: Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster', Genomics, vol. 82, no. 1, pp. 34-46. https://doi.org/10.1016/S0888-7543(03)00092-2
Deschamps, Stéphane ; Meyer, Jeffrey ; Chatterjee, Gouri ; Wang, Hong ; Lengyel, Peter ; Roe, Bruce A. / The mouse Ifi200 gene cluster : Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster. In: Genomics. 2003 ; Vol. 82, No. 1. pp. 34-46.
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