The mouse Ifi200 gene cluster: Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster

Stéphane Deschamps; Jeffrey Meyer; Gouri Chatterjee; Hong Wang; Peter Lengyel; Bruce A. Roe

doi:10.1016/S0888-7543(03)00092-2

The mouse Ifi200 gene cluster: Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster

Stéphane Deschamps, Jeffrey Meyer, Gouri Chatterjee, Hong Wang, Peter Lengyel, Bruce A. Roe

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30 Scopus citations

Abstract

The interferon-activatable Ifi200 gene cluster is located on mouse Chromosome 1q21-q23. We report here our analysis of two genomic regions encoding at least 10 closely related 200 family genes (Ifi201, Ifi202a, Ifi202b, Ifi202c, Ifi203a, Ifi203b, Ifi203c, Ifi203′, Ifi204, and Ifi204′) in 129/SvJ mice. Through a BAC-based sequencing approach, the exact structure and organization of these highly similar Ifi200 genes were obtained. A high degree of conservation (99% identity) was observed between Ifi202a and b and between Ifi203a and b. The presence of an additional transcribed region in intron 4 of Ifi203a and b suggests the possibility of alternative splicing, and a spliced variant of the Ifi204′ mRNA exhibits 91% sequence identity with a related but unmapped D3 mRNA. Comparative analysis of the mouse and human clusters indicates an absence of significant sequence conservation in noncoding sequences, suggesting that the 200 family emerged prior to human-mouse speciation and subsequently diverged after gene duplication.

Original language	English (US)
Pages (from-to)	34-46
Number of pages	13
Journal	Genomics
Volume	82
Issue number	1
DOIs	https://doi.org/10.1016/S0888-7543(03)00092-2
State	Published - Jul 1 2003

Keywords

Cluster
Comparison
Evolution
Interferon
Sequence

ASJC Scopus subject areas

Genetics

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10.1016/S0888-7543(03)00092-2

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title = "The mouse Ifi200 gene cluster: Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster",

abstract = "The interferon-activatable Ifi200 gene cluster is located on mouse Chromosome 1q21-q23. We report here our analysis of two genomic regions encoding at least 10 closely related 200 family genes (Ifi201, Ifi202a, Ifi202b, Ifi202c, Ifi203a, Ifi203b, Ifi203c, Ifi203′, Ifi204, and Ifi204′) in 129/SvJ mice. Through a BAC-based sequencing approach, the exact structure and organization of these highly similar Ifi200 genes were obtained. A high degree of conservation (99% identity) was observed between Ifi202a and b and between Ifi203a and b. The presence of an additional transcribed region in intron 4 of Ifi203a and b suggests the possibility of alternative splicing, and a spliced variant of the Ifi204′ mRNA exhibits 91% sequence identity with a related but unmapped D3 mRNA. Comparative analysis of the mouse and human clusters indicates an absence of significant sequence conservation in noncoding sequences, suggesting that the 200 family emerged prior to human-mouse speciation and subsequently diverged after gene duplication.",

keywords = "Cluster, Comparison, Evolution, Interferon, Sequence",

author = "St{\'e}phane Deschamps and Jeffrey Meyer and Gouri Chatterjee and Hong Wang and Peter Lengyel and Roe, {Bruce A.}",

note = "Funding Information: We thank Jonathon S. Coren for his technical help in sequencing the BAC clone mgs1-423c2. We also thank Steve Kenton, Hongshing Lai, and Jim White for their computational analysis; Rose Morales-Diaz and Mounir Elharam for their technical support; Elizabeth Vellali for typing a portion of the manuscript for publication; and the helpful comments of the anonymous reviewers. These studies were supported by a NIH research grant (HG02153) to B.A.R. and a NIH research grant (AI12320) to P.L.",

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AU - Deschamps, Stéphane

AU - Meyer, Jeffrey

AU - Chatterjee, Gouri

AU - Wang, Hong

AU - Lengyel, Peter

AU - Roe, Bruce A.

N1 - Funding Information: We thank Jonathon S. Coren for his technical help in sequencing the BAC clone mgs1-423c2. We also thank Steve Kenton, Hongshing Lai, and Jim White for their computational analysis; Rose Morales-Diaz and Mounir Elharam for their technical support; Elizabeth Vellali for typing a portion of the manuscript for publication; and the helpful comments of the anonymous reviewers. These studies were supported by a NIH research grant (HG02153) to B.A.R. and a NIH research grant (AI12320) to P.L.

PY - 2003/7/1

Y1 - 2003/7/1

N2 - The interferon-activatable Ifi200 gene cluster is located on mouse Chromosome 1q21-q23. We report here our analysis of two genomic regions encoding at least 10 closely related 200 family genes (Ifi201, Ifi202a, Ifi202b, Ifi202c, Ifi203a, Ifi203b, Ifi203c, Ifi203′, Ifi204, and Ifi204′) in 129/SvJ mice. Through a BAC-based sequencing approach, the exact structure and organization of these highly similar Ifi200 genes were obtained. A high degree of conservation (99% identity) was observed between Ifi202a and b and between Ifi203a and b. The presence of an additional transcribed region in intron 4 of Ifi203a and b suggests the possibility of alternative splicing, and a spliced variant of the Ifi204′ mRNA exhibits 91% sequence identity with a related but unmapped D3 mRNA. Comparative analysis of the mouse and human clusters indicates an absence of significant sequence conservation in noncoding sequences, suggesting that the 200 family emerged prior to human-mouse speciation and subsequently diverged after gene duplication.

AB - The interferon-activatable Ifi200 gene cluster is located on mouse Chromosome 1q21-q23. We report here our analysis of two genomic regions encoding at least 10 closely related 200 family genes (Ifi201, Ifi202a, Ifi202b, Ifi202c, Ifi203a, Ifi203b, Ifi203c, Ifi203′, Ifi204, and Ifi204′) in 129/SvJ mice. Through a BAC-based sequencing approach, the exact structure and organization of these highly similar Ifi200 genes were obtained. A high degree of conservation (99% identity) was observed between Ifi202a and b and between Ifi203a and b. The presence of an additional transcribed region in intron 4 of Ifi203a and b suggests the possibility of alternative splicing, and a spliced variant of the Ifi204′ mRNA exhibits 91% sequence identity with a related but unmapped D3 mRNA. Comparative analysis of the mouse and human clusters indicates an absence of significant sequence conservation in noncoding sequences, suggesting that the 200 family emerged prior to human-mouse speciation and subsequently diverged after gene duplication.

KW - Cluster

KW - Comparison

KW - Evolution

KW - Interferon

KW - Sequence

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The mouse Ifi200 gene cluster: Genomic sequence, analysis, and comparison with the human HIN-200 gene cluster

Abstract

Keywords

ASJC Scopus subject areas

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