The network of DAB2IP-miR-138 in regulating drug resistance of renal cell carcinoma associated with stem-like phenotypes

Eun Jin Yun, Jiancheng Zhou, Chun Jung Lin, Shan Xu, John Santoyo, Elizabeth Hernandez, Chih Ho Lai, Ho Lin, Dalin He, Jer Tsong Hsieh

Research output: Contribution to journalArticlepeer-review

12 Scopus citations

Abstract

Targeted therapy is a standard of care for metastatic renal cell carcinoma (RCC) but the response rate is not overwhelmed, which only prolongs a short survival of patients due to the onset of therapeutic resistance. Although the mechanisms are not fully understood, the presence of cancer initiating cells (CIC) may underlie the drug resistance. Nevertheless, identifying CIC phenotypes with its biomarkers in RCC appear to be diverse and controversial from many reports. In this study, we took a different approach to focus on the regulatory mechanism in RCC-CIC and unveil DAB2IP-mediated miR-138 expression that plays a critical role in modulating stem-like phenotypes in RCC via targeting the ABC transporter (ABCA13) as well as oncogenic histone methyltransferase EZH2 while down regulation of miR-138 gene expression in RCC is due to epigenetic gene silencing by DNA methyltransferase 1 (DNMT1). We also characterize the individual mechanism by which ABCA13 in RCC-CIC contributes to its drug resistance and. EZH2 maintain stem-like phenotypes. Noticeably, elevated expression of ABCA13 and EZH2 is correlated with overall survival of RCC patients, which can be used as potential prognostic markers. Taken together, this study demonstrates a potent and unique pathway of DAB2IP-mediated miR-138 in modulating CIC phenotypes during RCC progression and also offers a new therapeutic strategy of targeting drug resistant RCC.

Original languageEnglish (US)
Pages (from-to)66975-66986
Number of pages12
JournalOncotarget
Volume8
Issue number40
DOIs
StatePublished - May 9 2017

Keywords

  • Cancer stem cell
  • DAB2IP
  • Drug resistance
  • MiR-138

ASJC Scopus subject areas

  • Oncology

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