The noncoding RNA, miR-126, suppresses the growth of neoplastic cells by targeting phosphatidylinositol 3-kinase signaling and is frequently lost in colon cancers

Chunguang Guo, Jerome F. Sah, Lydia Beard, James K V Willson, Sanford D. Markowitz, Kishore Guda

Research output: Contribution to journalArticle

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Abstract

MicroRNAs (miRNA/miR) are a class of small noncoding RNAs implicated in the pathogenesis of various malignancies. In the current study, using micro(RNA) arrays, we found a ubiquitous loss of miR-126 expression in colon cancer lines when compared to normal human colon epithelia. Reconstitution of miR-126 in colon cancer cells resulted in a significant growth reduction as evidenced in clonogenic assays. A search for miR-126 gene targets revealed p85β, a regulatory subunit involved in stabilizing and propagating the phosphatidylinositol 3-kinase (PI3K) signal, as one of the potential substrates. Restoration of miR126 in cancer cells induced a ≥3-fold reduction in p85ß protein levels, with no concomitant change in p85αa, a gene that is functionally related to p85β but not a supposed target of miR-126. Additionally, using reporter constructs, we show that the p85β-3′ untranslated region is directly targeted by miR-126. Furthermore, this miR-126 mediated reduction of p85β was accompanied by a substantial reduction in phosphorylated AKT levels in the cancer cells, suggesting an impairment in PI3K signaling. Finally, in a panel of matched normal colon and primary colon tumors, each of the tumors demonstrated miR-126 down-regulation together with an increase in the p85β protein level. Taken together, we propose that miR-126 regulates PI3K signaling partly by targeting p85β, and that the loss of miR-126 may provide a selective growth advantage during colon carcinogenesis.

Original languageEnglish (US)
Pages (from-to)939-946
Number of pages8
JournalGenes Chromosomes and Cancer
Volume47
Issue number11
DOIs
StatePublished - Nov 2008

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Phosphatidylinositol 3-Kinase
Untranslated RNA
Colonic Neoplasms
Colon
MicroRNAs
Growth
Neoplasms
Small Untranslated RNA
3' Untranslated Regions
Genes
Carcinogenesis
Proteins
Down-Regulation
Epithelium

ASJC Scopus subject areas

  • Cancer Research
  • Genetics

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The noncoding RNA, miR-126, suppresses the growth of neoplastic cells by targeting phosphatidylinositol 3-kinase signaling and is frequently lost in colon cancers. / Guo, Chunguang; Sah, Jerome F.; Beard, Lydia; Willson, James K V; Markowitz, Sanford D.; Guda, Kishore.

In: Genes Chromosomes and Cancer, Vol. 47, No. 11, 11.2008, p. 939-946.

Research output: Contribution to journalArticle

Guo, Chunguang ; Sah, Jerome F. ; Beard, Lydia ; Willson, James K V ; Markowitz, Sanford D. ; Guda, Kishore. / The noncoding RNA, miR-126, suppresses the growth of neoplastic cells by targeting phosphatidylinositol 3-kinase signaling and is frequently lost in colon cancers. In: Genes Chromosomes and Cancer. 2008 ; Vol. 47, No. 11. pp. 939-946.
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