The osmotic-1 locus of Neurospora crassa encodes a putative histidine kinase similar to osmosensors of bacteria and yeast

Marc M. Schumacher, Carol S. Enderlin, Claude P. Selitrennikoff

Research output: Contribution to journalArticle

90 Scopus citations

Abstract

Osmotically sensitive mutants of Neurospora crassa are unable to grow on medium supplemented with 4% NaCl, have altered morphologies and cell-wall compositions, and are resistant to dicarboximide fungicides. Osmotic-1 (os- 1) mutants have a unique characteristic of forming protoplasts that grow and divide in specialized liquid medium, suggesting that the os-1+ gene product is important for cell-wall assembly. A cosmid containing the os-1+ locus of N. crassa, isolated from a genomic cosmid library by chromosomal walk from a closely linked gene, was used to subclone the ox-1+ gene by functional complementation of an as-1 mutant. Analysis of the sequence of complementing DNA predicts that os-1+ encodes a predicted protein similar to sensor- histidine kinases of bacteria and a yeast osmosensor-histidine kinase. Importantly, the predicted os-1+ protein is identical to the N. crassa nik- 1 predicted protein that was identified by using polymerase chain reaction primers directed against histidine kinase consensus DNA sequences. Our results indicate that nik-1 and os-1 encode the same osmosensing histidine kinase that plays an important role in the regulation of cell-wall assembly and, probably, other cell responses to changes in external osmolarity.

Original languageEnglish (US)
Pages (from-to)340-347
Number of pages8
JournalCurrent Microbiology
Volume34
Issue number6
DOIs
StatePublished - Jun 1 1997

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology

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