The proportion of glycosylated prolactin in serum is decreased in hyperprolactinemic states

Ibrahim A. Hashim, Roger Aston, Joan Butler, Alan M. McGregor, Caroline R. Smith, Michael Norman

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

The proportion of serum PRL that is glycosylated has been determined, and the effects of physiological and pathological hyperprolactinaemia on this proportion have been examined. Glycosylated and nonglycosylated PRL were immunoprecipitated from serum and subjected to polyacrylamide gel electrophoresis under reducing and dissociating conditions. Separated proteins were then transferred to nitrocellulose paper by electroblotting and detected immunologically with anti-PRL antiserum and 125I-labeled protein-A, followed by autoradiography. The proportion of total monomeric PRL present in the glycosylated form was then estimated by densitometric scanning of autoradiograms. In normal individuals glycosylated PRL was predominant, accounting for about 72% of the circulating monomeric PRL. This proportion was markedly decreased (ranging from undetectable to about 60%) in the serum of women who were pregnant or were lactating postpartum and also in patients with hyperprolactinemia caused by a pituitary tumor. The results suggest that under basal conditions the majority of PRL secreted from the pituitary is glycosylated, but with physiological or pathological hyperprolactinemia the capacity for glycosylation is exceeded. (J Clin Endocrinol Metab 71: 111-115, 1990).

Original languageEnglish (US)
Pages (from-to)111-115
Number of pages5
JournalJournal of Clinical Endocrinology and Metabolism
Volume71
Issue number1
StatePublished - Jul 1990

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Glycosylation
Hyperprolactinemia
Collodion
Staphylococcal Protein A
Electrophoresis
Tumors
Immune Sera
Scanning
Serum
Proteins
Pituitary Neoplasms
Autoradiography
Postpartum Period
Polyacrylamide Gel Electrophoresis
polyacrylamide gels
glycosylated prolactin

ASJC Scopus subject areas

  • Biochemistry
  • Endocrinology, Diabetes and Metabolism

Cite this

The proportion of glycosylated prolactin in serum is decreased in hyperprolactinemic states. / Hashim, Ibrahim A.; Aston, Roger; Butler, Joan; McGregor, Alan M.; Smith, Caroline R.; Norman, Michael.

In: Journal of Clinical Endocrinology and Metabolism, Vol. 71, No. 1, 07.1990, p. 111-115.

Research output: Contribution to journalArticle

Hashim, Ibrahim A. ; Aston, Roger ; Butler, Joan ; McGregor, Alan M. ; Smith, Caroline R. ; Norman, Michael. / The proportion of glycosylated prolactin in serum is decreased in hyperprolactinemic states. In: Journal of Clinical Endocrinology and Metabolism. 1990 ; Vol. 71, No. 1. pp. 111-115.
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abstract = "The proportion of serum PRL that is glycosylated has been determined, and the effects of physiological and pathological hyperprolactinaemia on this proportion have been examined. Glycosylated and nonglycosylated PRL were immunoprecipitated from serum and subjected to polyacrylamide gel electrophoresis under reducing and dissociating conditions. Separated proteins were then transferred to nitrocellulose paper by electroblotting and detected immunologically with anti-PRL antiserum and 125I-labeled protein-A, followed by autoradiography. The proportion of total monomeric PRL present in the glycosylated form was then estimated by densitometric scanning of autoradiograms. In normal individuals glycosylated PRL was predominant, accounting for about 72{\%} of the circulating monomeric PRL. This proportion was markedly decreased (ranging from undetectable to about 60{\%}) in the serum of women who were pregnant or were lactating postpartum and also in patients with hyperprolactinemia caused by a pituitary tumor. The results suggest that under basal conditions the majority of PRL secreted from the pituitary is glycosylated, but with physiological or pathological hyperprolactinemia the capacity for glycosylation is exceeded. (J Clin Endocrinol Metab 71: 111-115, 1990).",
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