The relationship of doxorubicin binding to membrane lipids with drug resistance

Sanjay Awasthi, Rajendra Sharma, Yogesh C. Awasthi, James A. Belli, Eugene P. Frenkel

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

Doxorubicin (DOX, Adriamycin) binds with high affinity to cellular membranes inflicting multiple lesions which are believed to be important in DOX-mediated neoplastic cell death. Using fluorescence and radioactive [14-14C-14]DOX assays for DOX, we have measured the partitioning of DOX between the cytosolic and membrane fractions of erythrocytes and of DOX-sensitive (V-79) and resistant (LZ) Chinese hamster lung fibroblast cells. In both erythrocytes and fibroblasts, a significant fraction of DOX was associated with the membrane fraction. More significantly, the quantity of lipid-bound DOX in the fibroblasts correlated with the cell's susceptibility to DOX. The significance of these findings in the context of existing knowledge about DOX-membrane interactions is discussed.

Original languageEnglish (US)
Pages (from-to)109-116
Number of pages8
JournalCancer Letters
Volume63
Issue number2
DOIs
StatePublished - Apr 15 1992

Fingerprint

Membrane Lipids
Drug Resistance
Doxorubicin
Fibroblasts
Membranes
Erythrocyte Membrane
Cricetulus
Cell Death
Erythrocytes
Fluorescence
Lipids
Lung

Keywords

  • Adriamycin
  • anthracycline
  • Chinese hamster lung
  • doxorubicin
  • drug resistance
  • fibroblast cell lines
  • subcellular partitioning of DOX
  • V-79 and LZ
  • [14-C]doxorubicin

ASJC Scopus subject areas

  • Cancer Research
  • Molecular Biology
  • Oncology

Cite this

Awasthi, S., Sharma, R., Awasthi, Y. C., Belli, J. A., & Frenkel, E. P. (1992). The relationship of doxorubicin binding to membrane lipids with drug resistance. Cancer Letters, 63(2), 109-116. https://doi.org/10.1016/0304-3835(92)90060-9

The relationship of doxorubicin binding to membrane lipids with drug resistance. / Awasthi, Sanjay; Sharma, Rajendra; Awasthi, Yogesh C.; Belli, James A.; Frenkel, Eugene P.

In: Cancer Letters, Vol. 63, No. 2, 15.04.1992, p. 109-116.

Research output: Contribution to journalArticle

Awasthi, S, Sharma, R, Awasthi, YC, Belli, JA & Frenkel, EP 1992, 'The relationship of doxorubicin binding to membrane lipids with drug resistance', Cancer Letters, vol. 63, no. 2, pp. 109-116. https://doi.org/10.1016/0304-3835(92)90060-9
Awasthi, Sanjay ; Sharma, Rajendra ; Awasthi, Yogesh C. ; Belli, James A. ; Frenkel, Eugene P. / The relationship of doxorubicin binding to membrane lipids with drug resistance. In: Cancer Letters. 1992 ; Vol. 63, No. 2. pp. 109-116.
@article{c5304f2a80ba4ef2970be1e7d4ed9d5a,
title = "The relationship of doxorubicin binding to membrane lipids with drug resistance",
abstract = "Doxorubicin (DOX, Adriamycin) binds with high affinity to cellular membranes inflicting multiple lesions which are believed to be important in DOX-mediated neoplastic cell death. Using fluorescence and radioactive [14-14C-14]DOX assays for DOX, we have measured the partitioning of DOX between the cytosolic and membrane fractions of erythrocytes and of DOX-sensitive (V-79) and resistant (LZ) Chinese hamster lung fibroblast cells. In both erythrocytes and fibroblasts, a significant fraction of DOX was associated with the membrane fraction. More significantly, the quantity of lipid-bound DOX in the fibroblasts correlated with the cell's susceptibility to DOX. The significance of these findings in the context of existing knowledge about DOX-membrane interactions is discussed.",
keywords = "Adriamycin, anthracycline, Chinese hamster lung, doxorubicin, drug resistance, fibroblast cell lines, subcellular partitioning of DOX, V-79 and LZ, [14-C]doxorubicin",
author = "Sanjay Awasthi and Rajendra Sharma and Awasthi, {Yogesh C.} and Belli, {James A.} and Frenkel, {Eugene P.}",
year = "1992",
month = "4",
day = "15",
doi = "10.1016/0304-3835(92)90060-9",
language = "English (US)",
volume = "63",
pages = "109--116",
journal = "Cancer Letters",
issn = "0304-3835",
publisher = "Elsevier Ireland Ltd",
number = "2",

}

TY - JOUR

T1 - The relationship of doxorubicin binding to membrane lipids with drug resistance

AU - Awasthi, Sanjay

AU - Sharma, Rajendra

AU - Awasthi, Yogesh C.

AU - Belli, James A.

AU - Frenkel, Eugene P.

PY - 1992/4/15

Y1 - 1992/4/15

N2 - Doxorubicin (DOX, Adriamycin) binds with high affinity to cellular membranes inflicting multiple lesions which are believed to be important in DOX-mediated neoplastic cell death. Using fluorescence and radioactive [14-14C-14]DOX assays for DOX, we have measured the partitioning of DOX between the cytosolic and membrane fractions of erythrocytes and of DOX-sensitive (V-79) and resistant (LZ) Chinese hamster lung fibroblast cells. In both erythrocytes and fibroblasts, a significant fraction of DOX was associated with the membrane fraction. More significantly, the quantity of lipid-bound DOX in the fibroblasts correlated with the cell's susceptibility to DOX. The significance of these findings in the context of existing knowledge about DOX-membrane interactions is discussed.

AB - Doxorubicin (DOX, Adriamycin) binds with high affinity to cellular membranes inflicting multiple lesions which are believed to be important in DOX-mediated neoplastic cell death. Using fluorescence and radioactive [14-14C-14]DOX assays for DOX, we have measured the partitioning of DOX between the cytosolic and membrane fractions of erythrocytes and of DOX-sensitive (V-79) and resistant (LZ) Chinese hamster lung fibroblast cells. In both erythrocytes and fibroblasts, a significant fraction of DOX was associated with the membrane fraction. More significantly, the quantity of lipid-bound DOX in the fibroblasts correlated with the cell's susceptibility to DOX. The significance of these findings in the context of existing knowledge about DOX-membrane interactions is discussed.

KW - Adriamycin

KW - anthracycline

KW - Chinese hamster lung

KW - doxorubicin

KW - drug resistance

KW - fibroblast cell lines

KW - subcellular partitioning of DOX

KW - V-79 and LZ

KW - [14-C]doxorubicin

UR - http://www.scopus.com/inward/record.url?scp=0026506407&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0026506407&partnerID=8YFLogxK

U2 - 10.1016/0304-3835(92)90060-9

DO - 10.1016/0304-3835(92)90060-9

M3 - Article

C2 - 1562987

AN - SCOPUS:0026506407

VL - 63

SP - 109

EP - 116

JO - Cancer Letters

JF - Cancer Letters

SN - 0304-3835

IS - 2

ER -