The Salmonella SpiC protein targets the mammalian Hook3 protein function to alter cellular trafficking

Yoram Shotland, Helmut Krämer, Eduardo A. Groisman

Research output: Contribution to journalArticlepeer-review

51 Scopus citations

Abstract

The Salmonella SpiC protein is secreted into the cytosol of macrophages via a unique type III secretion system that functions intracellularly to translocate proteins across the phagosomal membrane. The SpiC protein is required for survival within macrophages and inhibition of phagosome-lysosome fusion in vivo, and it is sufficient to inhibit endosome-endosome fusion in vitro. Here, we establish that SpiC targets the function of Hook3, a mammalian protein implicated in cellular trafficking. Purified GST-SpiC pulled down Hook3 from murine macrophages, and anti-Hook3 antibodies precipitated SpiC from the cytosol of Salmonella-infected macrophages. Expression of the spiC gene disrupted Golgi morphology in Vero cells and altered the distribution of lysosomes in macrophages, mimicking the phenotype of cells expressing a hook3 dominant-negative mutant. By inactivating Hook3 function, the SpiC protein may alter the lysosome network and prevent phagosome-lysosome fusion.

Original languageEnglish (US)
Pages (from-to)1565-1576
Number of pages12
JournalMolecular Microbiology
Volume49
Issue number6
DOIs
StatePublished - Sep 2003

ASJC Scopus subject areas

  • Microbiology
  • Molecular Biology

Fingerprint Dive into the research topics of 'The Salmonella SpiC protein targets the mammalian Hook3 protein function to alter cellular trafficking'. Together they form a unique fingerprint.

Cite this