The Schizosaccharomyces pombe pka1 gene, encoding a homolog of cAMP-dependent protein kinase

Gang Yu, Jiahua Li, Dalian Young

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

We have isolated 16 independent Schizosaccharomyces pombe cDNA clones that suppress the temperature-sensitive (ts) phenotype of a Saccharomyces cerevisiae strain containing the dominant-negative (ras2)val19ala22 allele. Fourteen of these cDNAs encode Sz. pombe Rasl. The other two clones encode the C-terminal region of a protein we have named Pka1. We have cloned the pka1 gene from a Sz. pombe genomic library. It contains an uninterrupted open reading frame encoding a 512-amino-acid (aa) protein. The C-terminal region (aa 200-512) of Pka1 is 51-63% identical to cAMP-dependent protein kinase (Pka) catalytic subunits from other eukaryotes. Production of Pkal suppresses the ts phenotypes exhibited by Sa. cerevisiae ras1-ras2ts or cyr1ts strains. Furthermore, overproduction of Pka1 in Sz. pombe results in a sterile phenotype and an abnormal morphology similar to that exhibited by cells in which the cAMP pathway is constitutively activated. These observations suggest that pka1 encodes the Sz. pombe Pka catalytic subunit.

Original languageEnglish (US)
Pages (from-to)215-220
Number of pages6
JournalGene
Volume151
Issue number1-2
DOIs
StatePublished - Dec 30 1994

Fingerprint

Schizosaccharomyces
Cyclic AMP-Dependent Protein Kinases
Phenotype
Saccharomyces cerevisiae
Catalytic Domain
Complementary DNA
Clone Cells
Genes
Amino Acids
Temperature
Genomic Library
Eukaryota
Protein Kinases
Open Reading Frames
Proteins
Alleles

Keywords

  • conjugation
  • mating
  • meiosis
  • Ras
  • Recombinant DNA
  • sporulation

ASJC Scopus subject areas

  • Genetics

Cite this

The Schizosaccharomyces pombe pka1 gene, encoding a homolog of cAMP-dependent protein kinase. / Yu, Gang; Li, Jiahua; Young, Dalian.

In: Gene, Vol. 151, No. 1-2, 30.12.1994, p. 215-220.

Research output: Contribution to journalArticle

Yu, Gang ; Li, Jiahua ; Young, Dalian. / The Schizosaccharomyces pombe pka1 gene, encoding a homolog of cAMP-dependent protein kinase. In: Gene. 1994 ; Vol. 151, No. 1-2. pp. 215-220.
@article{9de83a48676f40c0a745ef409d1e8b31,
title = "The Schizosaccharomyces pombe pka1 gene, encoding a homolog of cAMP-dependent protein kinase",
abstract = "We have isolated 16 independent Schizosaccharomyces pombe cDNA clones that suppress the temperature-sensitive (ts) phenotype of a Saccharomyces cerevisiae strain containing the dominant-negative (ras2)val19ala22 allele. Fourteen of these cDNAs encode Sz. pombe Rasl. The other two clones encode the C-terminal region of a protein we have named Pka1. We have cloned the pka1 gene from a Sz. pombe genomic library. It contains an uninterrupted open reading frame encoding a 512-amino-acid (aa) protein. The C-terminal region (aa 200-512) of Pka1 is 51-63{\%} identical to cAMP-dependent protein kinase (Pka) catalytic subunits from other eukaryotes. Production of Pkal suppresses the ts phenotypes exhibited by Sa. cerevisiae ras1-ras2ts or cyr1ts strains. Furthermore, overproduction of Pka1 in Sz. pombe results in a sterile phenotype and an abnormal morphology similar to that exhibited by cells in which the cAMP pathway is constitutively activated. These observations suggest that pka1 encodes the Sz. pombe Pka catalytic subunit.",
keywords = "conjugation, mating, meiosis, Ras, Recombinant DNA, sporulation",
author = "Gang Yu and Jiahua Li and Dalian Young",
year = "1994",
month = "12",
day = "30",
doi = "10.1016/0378-1119(94)90659-9",
language = "English (US)",
volume = "151",
pages = "215--220",
journal = "Gene",
issn = "0378-1119",
publisher = "Elsevier",
number = "1-2",

}

TY - JOUR

T1 - The Schizosaccharomyces pombe pka1 gene, encoding a homolog of cAMP-dependent protein kinase

AU - Yu, Gang

AU - Li, Jiahua

AU - Young, Dalian

PY - 1994/12/30

Y1 - 1994/12/30

N2 - We have isolated 16 independent Schizosaccharomyces pombe cDNA clones that suppress the temperature-sensitive (ts) phenotype of a Saccharomyces cerevisiae strain containing the dominant-negative (ras2)val19ala22 allele. Fourteen of these cDNAs encode Sz. pombe Rasl. The other two clones encode the C-terminal region of a protein we have named Pka1. We have cloned the pka1 gene from a Sz. pombe genomic library. It contains an uninterrupted open reading frame encoding a 512-amino-acid (aa) protein. The C-terminal region (aa 200-512) of Pka1 is 51-63% identical to cAMP-dependent protein kinase (Pka) catalytic subunits from other eukaryotes. Production of Pkal suppresses the ts phenotypes exhibited by Sa. cerevisiae ras1-ras2ts or cyr1ts strains. Furthermore, overproduction of Pka1 in Sz. pombe results in a sterile phenotype and an abnormal morphology similar to that exhibited by cells in which the cAMP pathway is constitutively activated. These observations suggest that pka1 encodes the Sz. pombe Pka catalytic subunit.

AB - We have isolated 16 independent Schizosaccharomyces pombe cDNA clones that suppress the temperature-sensitive (ts) phenotype of a Saccharomyces cerevisiae strain containing the dominant-negative (ras2)val19ala22 allele. Fourteen of these cDNAs encode Sz. pombe Rasl. The other two clones encode the C-terminal region of a protein we have named Pka1. We have cloned the pka1 gene from a Sz. pombe genomic library. It contains an uninterrupted open reading frame encoding a 512-amino-acid (aa) protein. The C-terminal region (aa 200-512) of Pka1 is 51-63% identical to cAMP-dependent protein kinase (Pka) catalytic subunits from other eukaryotes. Production of Pkal suppresses the ts phenotypes exhibited by Sa. cerevisiae ras1-ras2ts or cyr1ts strains. Furthermore, overproduction of Pka1 in Sz. pombe results in a sterile phenotype and an abnormal morphology similar to that exhibited by cells in which the cAMP pathway is constitutively activated. These observations suggest that pka1 encodes the Sz. pombe Pka catalytic subunit.

KW - conjugation

KW - mating

KW - meiosis

KW - Ras

KW - Recombinant DNA

KW - sporulation

UR - http://www.scopus.com/inward/record.url?scp=0028592569&partnerID=8YFLogxK

UR - http://www.scopus.com/inward/citedby.url?scp=0028592569&partnerID=8YFLogxK

U2 - 10.1016/0378-1119(94)90659-9

DO - 10.1016/0378-1119(94)90659-9

M3 - Article

C2 - 7828877

AN - SCOPUS:0028592569

VL - 151

SP - 215

EP - 220

JO - Gene

JF - Gene

SN - 0378-1119

IS - 1-2

ER -