The vast majority of CLA+ T cells are resident in normal skin

Rachael A. Clark, Benjamin Chong, Nina Mirchandani, Nooshin K. Brinster, Kei Ichi Yamanaka, Rebecca K. Dowgiert, Thomas S. Kupper

Research output: Contribution to journalArticle

441 Scopus citations

Abstract

There are T cells within normal, noninflamed skin that most likely conduct immunosurveillance and are implicated in the development of psoriasis. We isolated T cells from normal human skin using both established and novel methods. Skin resident T cells expressed high levels of CLA, CCR4, and CCR6, and a subset expressed CCR8 and CXCR6. Skin T cells had a remarkably diverse TCR repertoire and were mostly Th1 memory effector cells with smaller subsets of central memory, Th2, and functional T regulatory cells. We isolated a surprising number of nonexpanded T cells from normal skin. To validate this finding, we counted T cells in sections of normal skin and determined that there are ∼1 × 106 T cells/cm2 normal skin and an estimated 2 × 1010 T cells in the entire skin surface, nearly twice the number of T cells in the circulation. Moreover, we estimate that 98% of CLA + elector memory T cells are resident in normal skin under resting conditions. These findings demonstrate that there is a large pool of memory T cells in normal skin that can initiate and perpetuate immune reactions in the absence of T cell recruitment from the blood.

Original languageEnglish (US)
Pages (from-to)4431-4439
Number of pages9
JournalJournal of Immunology
Volume176
Issue number7
DOIs
StatePublished - Apr 1 2006

    Fingerprint

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

Cite this

Clark, R. A., Chong, B., Mirchandani, N., Brinster, N. K., Yamanaka, K. I., Dowgiert, R. K., & Kupper, T. S. (2006). The vast majority of CLA+ T cells are resident in normal skin. Journal of Immunology, 176(7), 4431-4439. https://doi.org/10.4049/jimmunol.176.7.4431