Three-dimensional live microscopy beyond the diffraction limit

Reto Fiolka

Research output: Contribution to journalArticle

7 Citations (Scopus)

Abstract

In fluorescence microscopy it has become possible to fundamentally overcome the diffraction limited resolution in all three spatial dimensions. However, to have the most impact in biological sciences, new optical microscopy techniques need to be compatible with live cell imaging: image acquisition has to be fast enough to capture cellular dynamics at the new resolution limit while light exposure needs to be minimized to prevent photo-toxic effects. With increasing spatial resolution, these requirements become more difficult to meet, even more so when volumetric imaging is performed. In this review, techniques that have been successfully applied to three-dimensional, super-resolution live microscopy are presented and their relative strengths and weaknesses are discussed.

Original languageEnglish (US)
Article number094002
JournalJournal of Optics (United Kingdom)
Volume15
Issue number9
DOIs
StatePublished - Sep 2013

Fingerprint

Microscopic examination
Diffraction
microscopy
Imaging techniques
Poisons
Image acquisition
Fluorescence microscopy
diffraction
Optical microscopy
acquisition
spatial resolution
fluorescence
requirements
cells

Keywords

  • 3D imaging
  • fluorescence microscopy
  • live cell imaging
  • structured illumination
  • Super-resolution microscopy

ASJC Scopus subject areas

  • Atomic and Molecular Physics, and Optics
  • Electronic, Optical and Magnetic Materials

Cite this

Three-dimensional live microscopy beyond the diffraction limit. / Fiolka, Reto.

In: Journal of Optics (United Kingdom), Vol. 15, No. 9, 094002, 09.2013.

Research output: Contribution to journalArticle

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