Three tandem HRDC domains have synergistic effect on the RecQ functions in Deinococcus radiodurans

Lifen Huang, Xiaoting Hua, Huiming Lu, Guanjun Gao, Bing Tian, Binghui Shen, Yuejin Hua

Research output: Contribution to journalArticle

26 Citations (Scopus)

Abstract

The RecQ family of DNA helicases performs essential functions in the maintenance of genomic stability in all organisms. In Deinococcus radiodurans, DR1289 is a special member of RecQ family with unique arrangement of three tandem HRDC domains in the C-terminus. A dr1289 mutant is hypersensitive to γ-irradiation, UV, H2O2 and mitomycin C. By complementing the dr1289 mutant with various domains of Dr1289 in vivo, we have determined that the helicase and all three HRDC domains are indispensable for complete DNA damage resistance. Using a continuous fluorescent dye-displacement assay, we investigated the optimal conditions for Dr1289 unwinding function at various concentrations of ATP and metal ions to show that the helicase activity is comparable to what observed in Escherichia coli RecQ. We also found that the helicase domain is necessary for the unwinding and ATPase activity and that the three tandem HRDC domains increase the efficiency of these activities. Based on these data, we propose that the C-terminus of Dr1289 has evolved in D. radiodurans to confront the types and amounts of DNA damage.

Original languageEnglish (US)
Pages (from-to)167-176
Number of pages10
JournalDNA Repair
Volume6
Issue number2
DOIs
StatePublished - Feb 4 2007

Fingerprint

Deinococcus
DNA Damage
RecQ Helicases
Genomic Instability
DNA
Mitomycin
Fluorescent Dyes
Escherichia coli
Metal ions
Adenosine Triphosphatases
Assays
Adenosine Triphosphate
Metals
Maintenance
Irradiation
Ions

Keywords

  • Deinococcus radiodurans
  • Helicase
  • HRDC
  • RecQ

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Three tandem HRDC domains have synergistic effect on the RecQ functions in Deinococcus radiodurans. / Huang, Lifen; Hua, Xiaoting; Lu, Huiming; Gao, Guanjun; Tian, Bing; Shen, Binghui; Hua, Yuejin.

In: DNA Repair, Vol. 6, No. 2, 04.02.2007, p. 167-176.

Research output: Contribution to journalArticle

Huang, Lifen ; Hua, Xiaoting ; Lu, Huiming ; Gao, Guanjun ; Tian, Bing ; Shen, Binghui ; Hua, Yuejin. / Three tandem HRDC domains have synergistic effect on the RecQ functions in Deinococcus radiodurans. In: DNA Repair. 2007 ; Vol. 6, No. 2. pp. 167-176.
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