Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice

A study of morphology, function, and T cell receptor expression

J. L. Nixon-Fulton, W. A. Kuziel, B. Santerse, P. R. Bergstresser, P. W. Tucker, R. E. Tigelaar

Research output: Contribution to journalArticle

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Abstract

We have evaluated the morphology, function, and TCR mRNA and DNA profiles of Thy-1+ epidermal cells (EC) derived from athymic (nude) mice. Based on these criteria we demonstrate that Thy-1+ EC in nude mice are predominantly round and angular and located in follicular epithelium, as compared to predominantly dendritic, interfollicular Thy-1+ EC found in normal CBA or BALB/c mice. Functionally, Thy-1+ EC from normal mice proliferate and elaborate IL-2 in response to stimulation by Con A; by contrast, nude Thy-1+ EC fail to proliferate or secrete IL-2 in response to Con A. Nude Thy-1+ EC proliferate markedly in response to low-dose IL-2 alone; Thy-1+ EC from normal mice give only modest proliferative responses to IL-2. Both populations of cells proliferate and elaborate IL-2 in response to PMA and calcium ionophore. Short-term cultured Thy-1+ EC from nude mice resemble Thy-1+ EC from normal animals in that they express no detectable functional TCR-α and β-RNA. Normal Thy-1+ EC express abundant levels of functional RNA for TCR-γ and δ-chains and for the CD3 δ-chain, whereas nude Thy-1+ EC produce no detectable TCR-δ and no CD3 δ-RNA and only truncated, presumably germ-line TCR-γ transcripts, as suggested by lack of hybridization with γ-V region probes and by lack of detectable rearrangements in the γ-genes. These phenotypic, functional, and genotypic characteristics of Thy-1+ EC from nude mice suggest that these cells are at a very early stage of T cell differentiation.

Original languageEnglish (US)
Pages (from-to)1897-1903
Number of pages7
JournalJournal of Immunology
Volume141
Issue number6
StatePublished - 1988

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T-Cell Antigen Receptor
Nude Mice
Thymus Gland
Interleukin-2
RNA
Calcium Ionophores
Gene Rearrangement
Germ Cells
Cell Differentiation
Epithelium

ASJC Scopus subject areas

  • Immunology

Cite this

Nixon-Fulton, J. L., Kuziel, W. A., Santerse, B., Bergstresser, P. R., Tucker, P. W., & Tigelaar, R. E. (1988). Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice: A study of morphology, function, and T cell receptor expression. Journal of Immunology, 141(6), 1897-1903.

Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice : A study of morphology, function, and T cell receptor expression. / Nixon-Fulton, J. L.; Kuziel, W. A.; Santerse, B.; Bergstresser, P. R.; Tucker, P. W.; Tigelaar, R. E.

In: Journal of Immunology, Vol. 141, No. 6, 1988, p. 1897-1903.

Research output: Contribution to journalArticle

Nixon-Fulton, JL, Kuziel, WA, Santerse, B, Bergstresser, PR, Tucker, PW & Tigelaar, RE 1988, 'Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice: A study of morphology, function, and T cell receptor expression', Journal of Immunology, vol. 141, no. 6, pp. 1897-1903.
Nixon-Fulton, J. L. ; Kuziel, W. A. ; Santerse, B. ; Bergstresser, P. R. ; Tucker, P. W. ; Tigelaar, R. E. / Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice : A study of morphology, function, and T cell receptor expression. In: Journal of Immunology. 1988 ; Vol. 141, No. 6. pp. 1897-1903.
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