TY - JOUR
T1 - Thy-1+ epidermal cells in nude mice are distinct from their counterparts in thymus-bearing mice
T2 - A study of morphology, function, and T cell receptor expression
AU - Nixon-Fulton, J. L.
AU - Kuziel, W. A.
AU - Santerse, B.
AU - Bergstresser, P. R.
AU - Tucker, P. W.
AU - Tigelaar, R. E.
N1 - Copyright:
Copyright 2004 Elsevier B.V., All rights reserved.
PY - 1988
Y1 - 1988
N2 - We have evaluated the morphology, function, and TCR mRNA and DNA profiles of Thy-1+ epidermal cells (EC) derived from athymic (nude) mice. Based on these criteria we demonstrate that Thy-1+ EC in nude mice are predominantly round and angular and located in follicular epithelium, as compared to predominantly dendritic, interfollicular Thy-1+ EC found in normal CBA or BALB/c mice. Functionally, Thy-1+ EC from normal mice proliferate and elaborate IL-2 in response to stimulation by Con A; by contrast, nude Thy-1+ EC fail to proliferate or secrete IL-2 in response to Con A. Nude Thy-1+ EC proliferate markedly in response to low-dose IL-2 alone; Thy-1+ EC from normal mice give only modest proliferative responses to IL-2. Both populations of cells proliferate and elaborate IL-2 in response to PMA and calcium ionophore. Short-term cultured Thy-1+ EC from nude mice resemble Thy-1+ EC from normal animals in that they express no detectable functional TCR-α and β-RNA. Normal Thy-1+ EC express abundant levels of functional RNA for TCR-γ and δ-chains and for the CD3 δ-chain, whereas nude Thy-1+ EC produce no detectable TCR-δ and no CD3 δ-RNA and only truncated, presumably germ-line TCR-γ transcripts, as suggested by lack of hybridization with γ-V region probes and by lack of detectable rearrangements in the γ-genes. These phenotypic, functional, and genotypic characteristics of Thy-1+ EC from nude mice suggest that these cells are at a very early stage of T cell differentiation.
AB - We have evaluated the morphology, function, and TCR mRNA and DNA profiles of Thy-1+ epidermal cells (EC) derived from athymic (nude) mice. Based on these criteria we demonstrate that Thy-1+ EC in nude mice are predominantly round and angular and located in follicular epithelium, as compared to predominantly dendritic, interfollicular Thy-1+ EC found in normal CBA or BALB/c mice. Functionally, Thy-1+ EC from normal mice proliferate and elaborate IL-2 in response to stimulation by Con A; by contrast, nude Thy-1+ EC fail to proliferate or secrete IL-2 in response to Con A. Nude Thy-1+ EC proliferate markedly in response to low-dose IL-2 alone; Thy-1+ EC from normal mice give only modest proliferative responses to IL-2. Both populations of cells proliferate and elaborate IL-2 in response to PMA and calcium ionophore. Short-term cultured Thy-1+ EC from nude mice resemble Thy-1+ EC from normal animals in that they express no detectable functional TCR-α and β-RNA. Normal Thy-1+ EC express abundant levels of functional RNA for TCR-γ and δ-chains and for the CD3 δ-chain, whereas nude Thy-1+ EC produce no detectable TCR-δ and no CD3 δ-RNA and only truncated, presumably germ-line TCR-γ transcripts, as suggested by lack of hybridization with γ-V region probes and by lack of detectable rearrangements in the γ-genes. These phenotypic, functional, and genotypic characteristics of Thy-1+ EC from nude mice suggest that these cells are at a very early stage of T cell differentiation.
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M3 - Article
C2 - 2902136
AN - SCOPUS:0023740847
SN - 0022-1767
VL - 141
SP - 1897
EP - 1903
JO - Journal of Immunology
JF - Journal of Immunology
IS - 6
ER -