Thy-1+ epidermal cells proliferate in response to Concanavalin A and interleukin 2

J. L. Nixon-Fulton, P. R. Bergstresser, R. E. Tigelaar

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33 Scopus citations

Abstract

Mouse epidermal cells (EC) are composed of at least two phenotypically discrete populations of cells that in epidermal sheets have a dendritic morphology: Ia+ Langerhans cells (LC) and dendritic, bone marrow-derived, Ia- cells that express Thy-1 antigen (Thy-1+ dEC). Thy-1+ dEC lack other typical T cell markers such as L3T4, Lyt-1, and Lyt-2; however, they do express Ly-5 and asialo GM1 in common with NK cells and certain other leukocytes. To investigate the functional capabilities of Thy-1+ dEC in vitro, cell suspensions prepared from trypsin-disaggregated sheets of mouse body wall epidermis were first enriched to 8 to 20% Ia+ and 20 to 40% Thy-1+ cells by centrifugation over Isolymph and then were cultured for 2 to 10 days with Concanavalin A (Con A) and/or partially purified rat IL 2. Con A-induced proliferation of EC was readily seen, with the maximal response occurring at a Con A concentration of 2.5 μg/ml on day 5 of culture. Con A responses were significantly enhanced by the continuous presence of 1 μg/ml indomethacin. Responses both in the presence and absence of Con A were significantly enhanced by the addition of 5 to 10 U/ml of partially purified rat IL 2; proliferation in cultures stimulated by both Con A and IL 2 continued to increase throughout the 10-day culture period. Culture of fluorescence-activated cell sorter (FACS)-separated EC suspensions revealed that Thy-1-depleted EC and irradiated Thy-1+ EC failed to proliferate in response to Con A and IL 2, whereas unirradiated purified Thy-1+ EC gave enhanced Con A- and IL 2-induced responses compared with the unseparated population. Finally, to distinguish between the proliferation of small numbers of mature peripheral T cells and that of Thy-1+ dEC, antibody and complement-depletion studies were conducted with an unusual monoclonal anti-Thy-1 reagent, 20-10-5S, and with the anti-T cell reagents, anti-L3T4 and anti-Lyt-2. Thy-1+ dEC, but not LC, express the 20-10-5S determinant; furthermore, in CBA (Thy-1.2) mice 20-10-5S reacts with Thy-1+ dEC, thymocytes, and peripheral T cells, whereas in AKR/J (Thy-1.1) mice, it reacts only with Thy-1+ dEC and thymocytes and not with peripheral T cells. Pretreatment of AKR/J EC with 20-10-5S and complement abolished the capacity of such cells to respond to Con A and to IL 2. Furthermore, pretreatment of CBA EC with anti-L3T4, anti-Lyt-2.1, and complement failed to diminish the proliferative response of these cells. These results suggest strongly that Thy-1+ dEC, which do not have a mature peripheral T cell phenytype, can proliferate in response to Con A and to IL 2. The ability to establish both short-term and long-term cell lines of Con A- and IL 2-stimulated Thy-1+ EC will facilitate analysis of the functional capabilities of these recently recognized cells residing within mouse epidermis.

Original languageEnglish (US)
Pages (from-to)2776-2786
Number of pages11
JournalJournal of Immunology
Volume136
Issue number8
StatePublished - 1986

ASJC Scopus subject areas

  • Immunology and Allergy
  • Immunology

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    Nixon-Fulton, J. L., Bergstresser, P. R., & Tigelaar, R. E. (1986). Thy-1+ epidermal cells proliferate in response to Concanavalin A and interleukin 2. Journal of Immunology, 136(8), 2776-2786.