Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression

Anice E. Thigpen, Richard I. Silver, Joseph M. Guileyardo, M. Linette Casey, Ohn D. McConnell, David W. Russell

Research output: Contribution to journalArticle

571 Citations (Scopus)

Abstract

The synthesis of dihydrotestosterone is catalyzed by steroid 5α-reductase isozymes, designated types 1 and 2. Mutation of type 2 results in male pseudohermaphroditism, in which the external genitalia are phenotypically female at birth. Two striking and unexplained features of this disorder are that external genitalia of affected males undergo virilization during puberty and that these individuals have less temporal hair regression. The tissue-specific and developmental expression patterns of the 5α-reductase isozymes were investigated by immunoblotting. The type 1 isozyme is not detectable in the fetus, is transiently expressed in newborn skin and scalp, and permanently expressed in skin from the time of puberty. There was no qualitative difference in 5α-reductase type 1 expression between adult balding vs. nonbalding scalp. The type 2 isozyme is transiently expressed in skin and scalp of newborns. Type 2 is the predominant isozyme detectable in fetal genital skin, male accessory sex glands, and in the prostate, including benign prostatic hyperplasia and prostate adenocarcinoma tissues. Both isozymes are expressed in the liver, but only after birth. These results are consistent with 5α-reductase type 1 being responsible for virilization in type 2-deficient subjects during puberty, and suggest that the type 2 isozyme may be an initiating factor in development of male pattern baldness.

Original languageEnglish (US)
Pages (from-to)903-910
Number of pages8
JournalJournal of Clinical Investigation
Volume92
Issue number2
StatePublished - Aug 1993

Fingerprint

Tissue Distribution
Isoenzymes
Oxidoreductases
Steroids
Puberty
Scalp
Virilism
Skin
Prostate
XY Disorders of Sex Development 46
Parturition
Male Genitalia
Female Genitalia
Dihydrotestosterone
Alopecia
Prostatic Hyperplasia
Immunoblotting
Hair
Adenocarcinoma
Fetus

Keywords

  • Benign prostatic hyperplasia
  • Dihydrotestosterone
  • Male pattern baldness
  • Prostate cancer
  • Sexual differentiation

ASJC Scopus subject areas

  • Medicine(all)

Cite this

Thigpen, A. E., Silver, R. I., Guileyardo, J. M., Casey, M. L., McConnell, O. D., & Russell, D. W. (1993). Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. Journal of Clinical Investigation, 92(2), 903-910.

Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. / Thigpen, Anice E.; Silver, Richard I.; Guileyardo, Joseph M.; Casey, M. Linette; McConnell, Ohn D.; Russell, David W.

In: Journal of Clinical Investigation, Vol. 92, No. 2, 08.1993, p. 903-910.

Research output: Contribution to journalArticle

Thigpen, AE, Silver, RI, Guileyardo, JM, Casey, ML, McConnell, OD & Russell, DW 1993, 'Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression', Journal of Clinical Investigation, vol. 92, no. 2, pp. 903-910.
Thigpen AE, Silver RI, Guileyardo JM, Casey ML, McConnell OD, Russell DW. Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. Journal of Clinical Investigation. 1993 Aug;92(2):903-910.
Thigpen, Anice E. ; Silver, Richard I. ; Guileyardo, Joseph M. ; Casey, M. Linette ; McConnell, Ohn D. ; Russell, David W. / Tissue distribution and ontogeny of steroid 5α-reductase isozyme expression. In: Journal of Clinical Investigation. 1993 ; Vol. 92, No. 2. pp. 903-910.
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