Tissue engineering bone using autologous progenitor cells in the peritoneum

Jinhui Shen, Ashwin Nair, Ramesh Saxena, Chengcheng Zhang, Joseph Borrelli, Liping Tang

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

Despite intensive research efforts, there remains a need for novel methods to improve the ossification of scaffolds for bone tissue engineering. Based on a common phenomenon and known pathological conditions of peritoneal membrane ossification following peritoneal dialysis, we have explored the possibility of regenerating ossified tissue in the peritoneum. Interestingly, in addition to inflammatory cells, we discovered a large number of multipotent mesenchymal stem cells (MSCs) in the peritoneal lavage fluid from mice with peritoneal catheter implants. The osteogenic potential of these peritoneal progenitor cells was demonstrated by their ability to easily infiltrate decalcified bone implants, produce osteocalcin and form mineralized bone in 8 weeks. Additionally, when poly(l-lactic acid) scaffolds loaded with bone morphogenetic protein-2 (a known osteogenic differentiation agent) were implanted into the peritoneum, signs of osteogenesis were seen within 8 weeks of implantation. The results of this investigation support the concept that scaffolds containing BMP-2 can stimulate the formation of bone in the peritoneum via directed autologous stem and progenitor cell responses.

Original languageEnglish (US)
Article numbere93514
JournalPLoS One
Volume9
Issue number3
DOIs
StatePublished - Mar 28 2014

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tissue engineering
peritoneum
Peritoneum
Tissue Engineering
bone formation
Tissue engineering
Osteogenesis
stem cells
Bone
Stem Cells
bones
Bone and Bones
prostheses
Scaffolds
Multipotent Stem Cells
Peritoneal Lavage
bone morphogenetic proteins
Bone Morphogenetic Protein 2
osteocalcin
Dialysis

ASJC Scopus subject areas

  • Agricultural and Biological Sciences(all)
  • Biochemistry, Genetics and Molecular Biology(all)
  • Medicine(all)

Cite this

Tissue engineering bone using autologous progenitor cells in the peritoneum. / Shen, Jinhui; Nair, Ashwin; Saxena, Ramesh; Zhang, Chengcheng; Borrelli, Joseph; Tang, Liping.

In: PLoS One, Vol. 9, No. 3, e93514, 28.03.2014.

Research output: Contribution to journalArticle

Shen, Jinhui ; Nair, Ashwin ; Saxena, Ramesh ; Zhang, Chengcheng ; Borrelli, Joseph ; Tang, Liping. / Tissue engineering bone using autologous progenitor cells in the peritoneum. In: PLoS One. 2014 ; Vol. 9, No. 3.
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