Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits

Robert R. Wolfe, Juquan Song, Jinchun Sun, Xiao Jun Zhang

Research output: Contribution to journalArticle

10 Citations (Scopus)

Abstract

Transfer RNA (tRNA)-charged amino acids are direct precursors of protein synthesis. Therefore, the amount and profile of amino acids in the aminoacyl-tRNA pool may be closely related to the rate of protein synthesis in the tissue. This study was designed to compare the aminoacyl-tRNA pools in liver and muscle, 2 distinct tissues with different rates of protein synthesis. Liver and muscle samples were taken from 6 rabbits and aminoacyl-tRNA was isolated with sequential acidphenol: chloroform extraction, followed by total RNA and tRNA purification. Amino acids in the aminoacyl-tRNA pool were measured by HPLC after deacylation. Liver contained 3.4 times more tRNA than muscle (585 ± 120 vs. 132 ± 11 μg of tRNA/g of tissue; P < 0.001). Overall tRNA charging was also greater in liver (14.22 ± 4.42 nmol of amino acids/mg of tRNA) than in muscle (7.00 ± 1.76 nmol of amino acids/mg of tRNA) (P < 0.05). The greater availability and charging efficiency of tRNA in liver as compared with muscle may influence the extent to which amino acid precursor availability regulates protein synthesis in these 2 tissues.

Original languageEnglish (US)
Pages (from-to)2333-2338
Number of pages6
JournalJournal of Nutrition
Volume137
Issue number11
StatePublished - Nov 2007

Fingerprint

transfer RNA
Transfer RNA
rabbits
Rabbits
Muscles
muscles
liver
Liver
amino acids
Amino Acids
protein synthesis
Transfer RNA Aminoacylation
aminoacyl transfer RNA
Proteins
chloroform
Protein Precursors
Chloroform
RNA
High Pressure Liquid Chromatography
tissues

ASJC Scopus subject areas

  • Medicine (miscellaneous)
  • Food Science

Cite this

Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits. / Wolfe, Robert R.; Song, Juquan; Sun, Jinchun; Zhang, Xiao Jun.

In: Journal of Nutrition, Vol. 137, No. 11, 11.2007, p. 2333-2338.

Research output: Contribution to journalArticle

Wolfe, RR, Song, J, Sun, J & Zhang, XJ 2007, 'Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits', Journal of Nutrition, vol. 137, no. 11, pp. 2333-2338.
Wolfe, Robert R. ; Song, Juquan ; Sun, Jinchun ; Zhang, Xiao Jun. / Total aminoacyl-transfer RNA pool is greater in liver than muscle in rabbits. In: Journal of Nutrition. 2007 ; Vol. 137, No. 11. pp. 2333-2338.
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