Transgene expression in Xenopus rods

Barry E. Knox, Charisse Schlueter, Brooke M. Sanger, Carla B. Green, Joseph C. Besharse

Research output: Contribution to journalArticle

90 Citations (Scopus)

Abstract

The photoreceptors of the vertebrate retina express a large number of proteins that are involved in the process of light transduction. These genes appear to be coordinately regulated at the level of transcription, with rod- and cone-specific isoforms (J. Hurley (1992) J. Bioenerg. Biomembr. 24, 219- 226). The mechanisms that regulate gene expression in a rod/cone-specific fashion have been difficult to address using traditional approaches and remain unknown. Regulation of the phototransduction proteins is medically important, since mutations in several of them cause retinal degeneration (P. Rosenreid and T. Dryja (1995) in: Molecular Genetics of Ocular Disease (J.L. Wiggs, Ed.), pp. 99-126, Wiley-Liss Inc.). An experimental system for rapidly producing retinas expressing a desired mutant would greatly facilitate investigations of retinal degeneration. We report here that transgenic frog embryos (K. Kroll and E. Amaya (1996) Development 122, 3173-3183) can be used to study cell-specific expression in the retina. We have used a 5.5 kb 5' upstream fragment from the Xenopus principal rod opsin gene (S. Batni et al. (1996) J. Biol. Chem. 271, 3179-3186) controlling a reporter gene, green fluorescent protein (GFP), to produce numerous independent transgenic Xenopus. We find that this construct drives expression only in the retina and pineal, which is apparent by 4 days post-nuclear injection. These are the first results using transgenic Xenopus for retinal promoter analysis and the potential for the expression in rod photoreceptors of proteins with dominant phenotypes.

Original languageEnglish (US)
Pages (from-to)117-121
Number of pages5
JournalFEBS Letters
Volume423
Issue number2
DOIs
StatePublished - Feb 20 1998

Fingerprint

Xenopus
Transgenes
Vertebrate Photoreceptor Cells
Retina
Genes
Retinal Degeneration
Cones
Rod Opsins
Proteins
Transcription
Light Signal Transduction
Green Fluorescent Proteins
Retinal Rod Photoreceptor Cells
Gene expression
Inborn Genetic Diseases
Eye Diseases
Protein Isoforms
Reporter Genes
Anura
Embryonic Development

Keywords

  • Gene expression
  • Photoreceptor
  • Promoter
  • Retina
  • Rhodopsin
  • Xenopus laevis

ASJC Scopus subject areas

  • Biochemistry
  • Biophysics
  • Molecular Biology

Cite this

Knox, B. E., Schlueter, C., Sanger, B. M., Green, C. B., & Besharse, J. C. (1998). Transgene expression in Xenopus rods. FEBS Letters, 423(2), 117-121. https://doi.org/10.1016/S0014-5793(98)00018-0

Transgene expression in Xenopus rods. / Knox, Barry E.; Schlueter, Charisse; Sanger, Brooke M.; Green, Carla B.; Besharse, Joseph C.

In: FEBS Letters, Vol. 423, No. 2, 20.02.1998, p. 117-121.

Research output: Contribution to journalArticle

Knox, BE, Schlueter, C, Sanger, BM, Green, CB & Besharse, JC 1998, 'Transgene expression in Xenopus rods', FEBS Letters, vol. 423, no. 2, pp. 117-121. https://doi.org/10.1016/S0014-5793(98)00018-0
Knox BE, Schlueter C, Sanger BM, Green CB, Besharse JC. Transgene expression in Xenopus rods. FEBS Letters. 1998 Feb 20;423(2):117-121. https://doi.org/10.1016/S0014-5793(98)00018-0
Knox, Barry E. ; Schlueter, Charisse ; Sanger, Brooke M. ; Green, Carla B. ; Besharse, Joseph C. / Transgene expression in Xenopus rods. In: FEBS Letters. 1998 ; Vol. 423, No. 2. pp. 117-121.
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