TY - JOUR
T1 - Transmission electron microscopy studies of Moraxella (Branhamella) catarrhalis
AU - Fitzgerald, Margaret
AU - Mulcahy, Riona
AU - Murphy, Susan
AU - Keane, Conor
AU - Coakley, Davis
AU - Scott, Thomas
N1 - Funding Information:
We wish to thank MS Ann Mynes, Electron Microscopy Unit, St James’s Hospital, Dublin and Mr David John, The Electron Microscopy Unit, Trinity College, Dublin for their help with the electron microscopy work. This work was supported by a Health Research Board of Ireland grant to M.F.
PY - 1999/1
Y1 - 1999/1
N2 - A trypsin-sensitive 200-kDa protein has been reported to be exclusively associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis. Transmission electron microscopy studies revealed that haemagglutination by M. catarrhalis to both human and rabbit erythrocytes was mediated by a trypsin-sensitive outer fibrillar coat. This fibrillar layer was absent on non-haemagglutinating isolates examined. Immuno-electron microscopy, using a polyclonal antiserum containing antibodies to the 200-kDa protein as a probe, showed that the 200-kDa protein is present on the outer fibrillar layer of the bacterium. These findings suggest that the haemagglutinin of M. catarrhalis is a 200-kDa protein present on the outer fibrillar coat. Copyright (C) 1999 Federation of European Microbiological Societies.
AB - A trypsin-sensitive 200-kDa protein has been reported to be exclusively associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis. Transmission electron microscopy studies revealed that haemagglutination by M. catarrhalis to both human and rabbit erythrocytes was mediated by a trypsin-sensitive outer fibrillar coat. This fibrillar layer was absent on non-haemagglutinating isolates examined. Immuno-electron microscopy, using a polyclonal antiserum containing antibodies to the 200-kDa protein as a probe, showed that the 200-kDa protein is present on the outer fibrillar layer of the bacterium. These findings suggest that the haemagglutinin of M. catarrhalis is a 200-kDa protein present on the outer fibrillar coat. Copyright (C) 1999 Federation of European Microbiological Societies.
KW - Adhesive interaction
KW - Electron microscopy
KW - Hemagglutination
KW - Immuno-electron microscopy
KW - Moraxella (Branhamella) catarrhalis
KW - Transmission electron microscopy
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U2 - 10.1016/S0928-8244(98)00121-7
DO - 10.1016/S0928-8244(98)00121-7
M3 - Article
C2 - 10030548
AN - SCOPUS:0032972334
SN - 0928-8244
VL - 23
SP - 57
EP - 66
JO - FEMS Immunology and Medical Microbiology
JF - FEMS Immunology and Medical Microbiology
IS - 1
ER -