Transmission electron microscopy studies of Moraxella (Branhamella) catarrhalis

Margaret Fitzgerald, Riona Mulcahy, Susan Murphy, Conor Keane, Davis Coakley, Thomas Scott

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A trypsin-sensitive 200-kDa protein has been reported to be exclusively associated with haemagglutinating isolates of Moraxella (Branhamella) catarrhalis. Transmission electron microscopy studies revealed that haemagglutination by M. catarrhalis to both human and rabbit erythrocytes was mediated by a trypsin-sensitive outer fibrillar coat. This fibrillar layer was absent on non-haemagglutinating isolates examined. Immuno-electron microscopy, using a polyclonal antiserum containing antibodies to the 200-kDa protein as a probe, showed that the 200-kDa protein is present on the outer fibrillar layer of the bacterium. These findings suggest that the haemagglutinin of M. catarrhalis is a 200-kDa protein present on the outer fibrillar coat. Copyright (C) 1999 Federation of European Microbiological Societies.

Original languageEnglish (US)
Pages (from-to)57-66
Number of pages10
JournalFEMS Immunology and Medical Microbiology
Volume23
Issue number1
DOIs
StatePublished - Jan 1 1999

Fingerprint

Moraxella (Branhamella) catarrhalis
Transmission Electron Microscopy
Trypsin
Proteins
Immunoelectron Microscopy
Hemagglutination
Hemagglutinins
Immune Sera
Erythrocytes
Rabbits
Bacteria
Antibodies

Keywords

  • Adhesive interaction
  • Electron microscopy
  • Hemagglutination
  • Immuno-electron microscopy
  • Moraxella (Branhamella) catarrhalis
  • Transmission electron microscopy

ASJC Scopus subject areas

  • Immunology and Allergy
  • Microbiology
  • Immunology
  • Microbiology (medical)
  • Infectious Diseases

Cite this

Transmission electron microscopy studies of Moraxella (Branhamella) catarrhalis. / Fitzgerald, Margaret; Mulcahy, Riona; Murphy, Susan; Keane, Conor; Coakley, Davis; Scott, Thomas.

In: FEMS Immunology and Medical Microbiology, Vol. 23, No. 1, 01.01.1999, p. 57-66.

Research output: Contribution to journalArticle

Fitzgerald, Margaret ; Mulcahy, Riona ; Murphy, Susan ; Keane, Conor ; Coakley, Davis ; Scott, Thomas. / Transmission electron microscopy studies of Moraxella (Branhamella) catarrhalis. In: FEMS Immunology and Medical Microbiology. 1999 ; Vol. 23, No. 1. pp. 57-66.
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