Transplantation of β cells from transgenic mice into nude athymic diabetic rats restores glucose regulation

Barry A. Hicks, Roland Stein, Shimon Efrat, Seth Grant, Douglas Hanahan, Achilles A. Demetriou

Research output: Contribution to journalArticle

13 Scopus citations

Abstract

We have shown that elevated plasma d-glucose levels in experimentally-induced diabetic nude athymic rats can be reduced by intraperitoneal transplantation of microcarrier-attached insulin producing β cells from the mouse pancreatic β cell line, β TC-1. The reduction in the level of hyperglycemia was observed as early as two days following cell transplantation and was associated with a concomitant increase in plasma insulin levels. β TC-1 cell transplanted diabetic rats had plasma d-glucose levels similar to those found in non-diabetic control animals and remained normoglycemic throughout the 39 day experimental period. The β TC-1 cell transplanted diabetic rats also had near normalization of body weight, food and water intake and of urine output when compared to control diabetic and non-diabetic rats. Similarly, they exhibited improved blood glucose clearance following intravenous d-glucose administration. These results suggest that β TC-1 cells regulate d-glucose homeostasis following transplantation into diabetic rat recipients in a manner similar to that of endogenous pancreatic β cells.

Original languageEnglish (US)
Pages (from-to)157-164
Number of pages8
JournalDiabetes Research and Clinical Practice
Volume14
Issue number3
DOIs
StatePublished - Dec 1991

Keywords

  • Cell therapy
  • Insulin-dependent diabetes mellitus
  • Rat
  • Streptozotocin induced diabetes
  • β cell transplantation

ASJC Scopus subject areas

  • Internal Medicine
  • Endocrinology, Diabetes and Metabolism
  • Endocrinology

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