Traumatic neuronal injury in vitro is attenuated by NMDA antagonists

Evelyn S. Tecoma, Hannelore Monyer, Mark P. Goldberg, Dennis W. Choi

Research output: Contribution to journalArticle

111 Scopus citations

Abstract

Pure traumatic neuronal injury was modeled in dispersed neocortical cell cultures derived from fetal mice. A plastic stylet was used to tear the neuronal and glial cell layer; medium oxygen content, pH, and glucose remained unchanged. Adjacent to this local disruption, many neurons developed acute swelling and went on to degenerate over the next day, but glia were relatively spared. If the same mechanical insult was delivered in the presence of the N-methyl-d-aspartate (NMDA) antagonists dextrorphan or d-2-amino-5-phosphonoval-erate, resultant neuronal degeneration was markedly reduced. The protective effect of these NMDA antagonists was concentration-dependent between 1 and 100 μM, with EC50 near 10 μM for both compounds. Present findings suggest that endogenous excitatory amino acids may participate significantly in the propagation of central neuronal cell loss in response to a purely mechanical insult.

Original languageEnglish (US)
Pages (from-to)1541-1545
Number of pages5
JournalNeuron
Volume2
Issue number6
DOIs
StatePublished - Jun 1989

ASJC Scopus subject areas

  • Neuroscience(all)

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