Trout myomaker contains 14 minisatellites and two sequence extensions but retains fusogenic function

Aurélie Landemaine, Andres Ramirez-Martinez, Olivier Monestier, Nathalie Sabin, Pierre Yves Rescan, Eric N Olson, Jean Charles Gabillard

Research output: Contribution to journalArticle

Abstract

The formation of new myofibers in vertebrates occurs by myoblast fusion and requires fusogenic activity of the muscle-specific membrane protein myomaker. Here, using in silico (BLAST) genome analyses, we show that the myomaker gene from trout includes 14 minisatellites, indicating that it has an unusual structure compared with those of other animal species. We found that the trout myomaker gene encodes a 434 –amino acid (aa) protein, in accordance with its apparent molecular mass (40 kDa) observed by immunoblotting. The first half of the trout myomaker protein (1–220 aa) is similar to the 221-aa mouse myomaker protein, whereas the second half (222–234 aa) does not correspond to any known motifs and arises from two protein extensions. The first extension (70 aa) apparently appeared with the radiation of the bony fish clade Euteleostei, whereas the second extension (up to 236 aa) is restricted to the superorder Protacanthopterygii (containing salmonids and pike) and corresponds to the insertion of minisatellites having a length of 30 nucleotides. According to gene expression analyses, trout myomaker expression is consistently associated with the formation of new myofibers during embryonic development, postlarval growth, and muscle regeneration. Using cell-mixing experiments, we observed that trout myomaker has retained the ability to drive the fusion of mouse fibroblasts with C2C12 myoblasts. Our work reveals that trout myomaker has fusogenic function despite containing two protein extensions.

Original languageEnglish (US)
Pages (from-to)6364-6374
Number of pages11
JournalJournal of Biological Chemistry
Volume294
Issue number16
DOIs
StatePublished - Jan 1 2019

Fingerprint

Minisatellite Repeats
Trout
Amino Acids
Genes
Myoblasts
Muscle
Proteins
Fusion reactions
Esocidae
Salmonidae
Muscles
Molecular mass
Fibroblasts
Immunoblotting
Gene expression
Computer Simulation
Fish
Embryonic Development
Vertebrates
Regeneration

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology
  • Cell Biology

Cite this

Landemaine, A., Ramirez-Martinez, A., Monestier, O., Sabin, N., Rescan, P. Y., Olson, E. N., & Gabillard, J. C. (2019). Trout myomaker contains 14 minisatellites and two sequence extensions but retains fusogenic function. Journal of Biological Chemistry, 294(16), 6364-6374. https://doi.org/10.1074/jbc.RA118.006047

Trout myomaker contains 14 minisatellites and two sequence extensions but retains fusogenic function. / Landemaine, Aurélie; Ramirez-Martinez, Andres; Monestier, Olivier; Sabin, Nathalie; Rescan, Pierre Yves; Olson, Eric N; Gabillard, Jean Charles.

In: Journal of Biological Chemistry, Vol. 294, No. 16, 01.01.2019, p. 6364-6374.

Research output: Contribution to journalArticle

Landemaine, A, Ramirez-Martinez, A, Monestier, O, Sabin, N, Rescan, PY, Olson, EN & Gabillard, JC 2019, 'Trout myomaker contains 14 minisatellites and two sequence extensions but retains fusogenic function', Journal of Biological Chemistry, vol. 294, no. 16, pp. 6364-6374. https://doi.org/10.1074/jbc.RA118.006047
Landemaine, Aurélie ; Ramirez-Martinez, Andres ; Monestier, Olivier ; Sabin, Nathalie ; Rescan, Pierre Yves ; Olson, Eric N ; Gabillard, Jean Charles. / Trout myomaker contains 14 minisatellites and two sequence extensions but retains fusogenic function. In: Journal of Biological Chemistry. 2019 ; Vol. 294, No. 16. pp. 6364-6374.
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