Trypanosoma brucei metabolism is under circadian control

Filipa Rijo-Ferreira, Daniel Pinto-Neves, Nuno L. Barbosa-Morais, Joseph S. Takahashi, Luisa M. Figueiredo

Research output: Contribution to journalArticle

20 Citations (Scopus)

Abstract

The Earth's rotation forced life to evolve under cyclic day and night environmental changes. To anticipate such daily cycles, prokaryote and eukaryote free-living organisms evolved intrinsic clocks that regulate physiological and behavioural processes. Daily rhythms have been observed in organisms living within hosts, such as parasites. Whether parasites have intrinsic molecular clocks or whether they simply respond to host rhythmic physiological cues remains unknown. Here, we show that Trypanosoma brucei, the causative agent of human sleeping sickness, has an intrinsic circadian clock that regulates its metabolism in two different stages of the life cycle. We found that, in vitro, ∼ 10% of genes in T. brucei are expressed with a circadian rhythm. The maximum expression of these genes occurs at two different phases of the day and may depend on a post-transcriptional mechanism. Circadian genes are enriched in cellular metabolic pathways and coincide with two peaks of intracellular adenosine triphosphate concentration. Moreover, daily changes in the parasite population lead to differences in suramin sensitivity, a drug commonly used to treat this infection. These results demonstrate that parasites have an intrinsic circadian clock that is independent of the host, and which regulates parasite biology throughout the day.

Original languageEnglish (US)
Article number17032
JournalNature Microbiology
Volume2
DOIs
StatePublished - Mar 13 2017

Fingerprint

Trypanosoma brucei brucei
Parasites
Circadian Clocks
Physiological Phenomena
Suramin
Circadian Rhythm
Metabolic Networks and Pathways
Life Cycle Stages
Eukaryota
Genes
Cues
Adenosine Triphosphate
Gene Expression
Infection
Pharmaceutical Preparations
Population

ASJC Scopus subject areas

  • Microbiology
  • Applied Microbiology and Biotechnology
  • Immunology
  • Microbiology (medical)
  • Cell Biology
  • Genetics

Cite this

Rijo-Ferreira, F., Pinto-Neves, D., Barbosa-Morais, N. L., Takahashi, J. S., & Figueiredo, L. M. (2017). Trypanosoma brucei metabolism is under circadian control. Nature Microbiology, 2, [17032]. https://doi.org/10.1038/nmicrobiol.2017.32

Trypanosoma brucei metabolism is under circadian control. / Rijo-Ferreira, Filipa; Pinto-Neves, Daniel; Barbosa-Morais, Nuno L.; Takahashi, Joseph S.; Figueiredo, Luisa M.

In: Nature Microbiology, Vol. 2, 17032, 13.03.2017.

Research output: Contribution to journalArticle

Rijo-Ferreira, F, Pinto-Neves, D, Barbosa-Morais, NL, Takahashi, JS & Figueiredo, LM 2017, 'Trypanosoma brucei metabolism is under circadian control', Nature Microbiology, vol. 2, 17032. https://doi.org/10.1038/nmicrobiol.2017.32
Rijo-Ferreira F, Pinto-Neves D, Barbosa-Morais NL, Takahashi JS, Figueiredo LM. Trypanosoma brucei metabolism is under circadian control. Nature Microbiology. 2017 Mar 13;2. 17032. https://doi.org/10.1038/nmicrobiol.2017.32
Rijo-Ferreira, Filipa ; Pinto-Neves, Daniel ; Barbosa-Morais, Nuno L. ; Takahashi, Joseph S. ; Figueiredo, Luisa M. / Trypanosoma brucei metabolism is under circadian control. In: Nature Microbiology. 2017 ; Vol. 2.
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