Tumor masses support naive T cell infiltration, activation, and differentiation into effectors

Elizabeth D. Thompson, Hilda L. Enriquez, Yang Xin Fu, Victor H. Engelhard

Research output: Contribution to journalArticle

112 Citations (Scopus)

Abstract

Studies of T cell responses to tumors have focused on the draining lymph node (LN) as the site of activation. We examined the tumor mass as a potential site of activation after adoptive transfer of naive tumor-specific CD8 T cells. Activated CD8 T cells were present in tumors within 24 h of adoptive transfer and proliferation of these cells was also evident 4-5 d later in mice treated with FTY720 to prevent infiltration of cells activated in LNs. To confirm that activation of these T cells occurred in the tumor and not the tumor-draining LNs, we used mice lacking LNs. Activated and proliferating tumor-infiltrating lymphocytes were evident in these mice 24 h and 4 d after naive cell transfer. T cells activated within tumors acquired effector function that was evident both ex vivo and in vivo. Both cross-presenting antigen presenting cells within the tumor and tumor cells directly presenting antigen activated these functional CD8 effectors. We conclude that tumors support the infiltration, activation, and effector differentiation of naive CD8 T cells, despite the presence of immunosuppressive mechanisms. Thus, targeting of T cell activation to tumors may present a tool in the development of cancer immunotherapy.

Original languageEnglish (US)
Pages (from-to)1791-1804
Number of pages14
JournalJournal of Experimental Medicine
Volume207
Issue number8
DOIs
StatePublished - Aug 2 2010

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T-Lymphocytes
Neoplasms
Adoptive Transfer
Antigen-Presenting Cells
Tumor-Infiltrating Lymphocytes
Immunosuppressive Agents
Immunotherapy
Lymph Nodes

ASJC Scopus subject areas

  • Immunology
  • Immunology and Allergy
  • Medicine(all)

Cite this

Tumor masses support naive T cell infiltration, activation, and differentiation into effectors. / Thompson, Elizabeth D.; Enriquez, Hilda L.; Fu, Yang Xin; Engelhard, Victor H.

In: Journal of Experimental Medicine, Vol. 207, No. 8, 02.08.2010, p. 1791-1804.

Research output: Contribution to journalArticle

Thompson, Elizabeth D. ; Enriquez, Hilda L. ; Fu, Yang Xin ; Engelhard, Victor H. / Tumor masses support naive T cell infiltration, activation, and differentiation into effectors. In: Journal of Experimental Medicine. 2010 ; Vol. 207, No. 8. pp. 1791-1804.
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