TY - JOUR
T1 - Two regions of homozygous deletion clusters at chromosome band 9p21 in human lung cancer
AU - Hamada, Kunihiro
AU - Kohno, Takashi
AU - Takahashi, Mina
AU - Yamazaki, Masaaki
AU - Tashiro, Hiroyuki
AU - Sugawara, Chiyo
AU - Ohwada, Susumu
AU - Sekido, Yoshitaka
AU - Minna, John D.
AU - Yokota, Jun
PY - 2000/3
Y1 - 2000/3
N2 - We examined 149 lung cancer cell lines for homozygous deletions using 24 DNA markers, which were mapped and ordered in chromosome band 9p21, to define the target regions for 9p21 deletions in human lung cancer. Homozygous deletions were detected in 39 (26%) cell lines and clustered at 2 independent regions. One was the region containing the p161CDKN2A/tumor suppressor gene, and this region was deleted in 32 (21%) cell lines. The other was the region containing D9S 171, which is the locus approximately 3 Mb proximal to the CDKN2A locus. This region, designated as the D9S171 region, was deleted in 18 (12%) cell lines. Seven of the 18 cell lines had identical minimum deletions of a 17,036 bp sequence located 20 kb distal to the D9S171 locus. However, such a deletion was also observed in the corresponding B-lymphoblastoid cell line from 1 of the 7 cell lines and in 5 (16%) of 32 noncancerous tissues, suggesting that the deletion was a genetic polymorphism. By considering this polymorphism, 11 (7%) cell lines still had deletions at the D9S171 region. Two NSCLC cell lines showed deletions at the D9S171 region and retentions of the CDKN2A locus. Furthermore, an NSCLC cell line showed discontinuous deletions including either the CDKN2A or D9S171 locus. Therefore, the region surrounding the D9S171 locus was defined as a another target region for the 9p21 deletions. It is possible that unknown tumor suppressor gene(s) are present in this chromosomal region.
AB - We examined 149 lung cancer cell lines for homozygous deletions using 24 DNA markers, which were mapped and ordered in chromosome band 9p21, to define the target regions for 9p21 deletions in human lung cancer. Homozygous deletions were detected in 39 (26%) cell lines and clustered at 2 independent regions. One was the region containing the p161CDKN2A/tumor suppressor gene, and this region was deleted in 32 (21%) cell lines. The other was the region containing D9S 171, which is the locus approximately 3 Mb proximal to the CDKN2A locus. This region, designated as the D9S171 region, was deleted in 18 (12%) cell lines. Seven of the 18 cell lines had identical minimum deletions of a 17,036 bp sequence located 20 kb distal to the D9S171 locus. However, such a deletion was also observed in the corresponding B-lymphoblastoid cell line from 1 of the 7 cell lines and in 5 (16%) of 32 noncancerous tissues, suggesting that the deletion was a genetic polymorphism. By considering this polymorphism, 11 (7%) cell lines still had deletions at the D9S171 region. Two NSCLC cell lines showed deletions at the D9S171 region and retentions of the CDKN2A locus. Furthermore, an NSCLC cell line showed discontinuous deletions including either the CDKN2A or D9S171 locus. Therefore, the region surrounding the D9S171 locus was defined as a another target region for the 9p21 deletions. It is possible that unknown tumor suppressor gene(s) are present in this chromosomal region.
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U2 - 10.1002/(SICI)1098-2264(200003)27:3<308::AID-GCC12>3.0.CO;2-W
DO - 10.1002/(SICI)1098-2264(200003)27:3<308::AID-GCC12>3.0.CO;2-W
M3 - Article
C2 - 10679921
AN - SCOPUS:0033971825
SN - 1045-2257
VL - 27
SP - 308
EP - 318
JO - Genes Chromosomes and Cancer
JF - Genes Chromosomes and Cancer
IS - 3
ER -