TY - JOUR
T1 - Type 1 and type 2 cytokine profiles in children exposed to or infected with vertically transmitted human immunodeficiency virus
AU - Lee, Bang Ning
AU - Lu, Jian Guo
AU - Kline, Mark W.
AU - Paul, Mary
AU - Doyle, Marilyn
AU - Kozinetz, Claudia
AU - Shearer, William T.
AU - Reuben, James M.
PY - 1996
Y1 - 1996
N2 - In human immunodeficiency virus (HIV)-infected adults, cytokine production profiles snitch from predominantly type 1 (interleukin-2 [IL-2] and gamma interferon [IFN-γ]) to type 2 (IL-4 and IL-10) cytokines with disease progression. To test this hypothesis in vertically HIV-infected children, we measured cytokine transcription and production in rapid progressors (RPs), seroreverters (SRs), and those children exposed to HIV in utero (P0s). Production of type 1 and type 2 cytokines was measured in peripheral blood mononuclear cell cultures of 8 SR, 25 P0, and 11 RP children. Unstimulated cultures, irrespective of infection and stage of disease, produced similar levels of IL-2, IFN-γ, IL-4, and IL-10. Upon stimulation with phytohemagglutinin (PHA) plus phorbol-12-myristate-13-acetate (PMA), RP children produced less IL-2 (P < 0.01) and IFN-γ (P < 0.02) than SR children and also expressed significantly less IFN-γ mRNA (P < 0.01) than SR children. RP children expressed significantly higher levels of IL-4 mRNA than P0 children (P < 0.03). There were no differences in the production of IL-10 by PHA-PMA-stimulated peripheral blood mononuclear cell cultures among the three groups of children. Our data with these pediatric patients suggest that a deficiency in mitogen-stimulated type 1 cytokine production and excess type 2 cytokine (IL-4) transcription correlate with disease progression. Additional studies with larger sample sizes are needed to test further the hypothesis of the type 1-to-type 2 cytokine switch in children infected with HIV.
AB - In human immunodeficiency virus (HIV)-infected adults, cytokine production profiles snitch from predominantly type 1 (interleukin-2 [IL-2] and gamma interferon [IFN-γ]) to type 2 (IL-4 and IL-10) cytokines with disease progression. To test this hypothesis in vertically HIV-infected children, we measured cytokine transcription and production in rapid progressors (RPs), seroreverters (SRs), and those children exposed to HIV in utero (P0s). Production of type 1 and type 2 cytokines was measured in peripheral blood mononuclear cell cultures of 8 SR, 25 P0, and 11 RP children. Unstimulated cultures, irrespective of infection and stage of disease, produced similar levels of IL-2, IFN-γ, IL-4, and IL-10. Upon stimulation with phytohemagglutinin (PHA) plus phorbol-12-myristate-13-acetate (PMA), RP children produced less IL-2 (P < 0.01) and IFN-γ (P < 0.02) than SR children and also expressed significantly less IFN-γ mRNA (P < 0.01) than SR children. RP children expressed significantly higher levels of IL-4 mRNA than P0 children (P < 0.03). There were no differences in the production of IL-10 by PHA-PMA-stimulated peripheral blood mononuclear cell cultures among the three groups of children. Our data with these pediatric patients suggest that a deficiency in mitogen-stimulated type 1 cytokine production and excess type 2 cytokine (IL-4) transcription correlate with disease progression. Additional studies with larger sample sizes are needed to test further the hypothesis of the type 1-to-type 2 cytokine switch in children infected with HIV.
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U2 - 10.1128/cdli.3.5.493-499.1996
DO - 10.1128/cdli.3.5.493-499.1996
M3 - Article
C2 - 8877124
AN - SCOPUS:0029836425
SN - 1071-412X
VL - 3
SP - 493
EP - 499
JO - Clinical and Diagnostic Laboratory Immunology
JF - Clinical and Diagnostic Laboratory Immunology
IS - 5
ER -