TY - JOUR
T1 - Type III Effector VopC Mediates Invasion for Vibrio Species
AU - Zhang, Lingling
AU - Krachler, Anne Marie
AU - Broberg, Christopher A.
AU - Li, Yan
AU - Mirzael, Hamid
AU - Gilpin, Christopher J.
AU - Orth, Kim
N1 - Funding Information:
For insightful discussions, critical reading, and/or generous supply of reagents, we thank N. Alto, T. Iida, T. Honda, L. McCarter, M. Phillips, V. Miller, J. Mekalanos, and the Orth lab. K.O., L.Z., C.A.B., and A.M.K. are supported by grants from NIH and the Welch Foundation (I-1561). K.O. is a Beckman Young Investigator, Burroughs Wellcome Investigator, and W.W. Caruth Biomedical Scholar.
PY - 2012/5/31
Y1 - 2012/5/31
N2 - Vibrio spp. are associated with infections caused by contaminated food and water. A type III secretion system (T3SS2) is a shared feature of all clinical isolates of . V. parahaemolyticus and some . V. cholerae strains. Despite its being responsible for enterotoxicity, no molecular mechanism has been determined for the T3SS2-dependent pathogenicity. Here, we show that although . Vibrio spp. are typically thought of as extracellular pathogens, the T3SS2 of . Vibrio mediates host cell invasion, vacuole formation, and replication of intracellular bacteria. The catalytically active effector VopC is critical for . Vibrio T3SS2-mediated invasion. There are other marine bacteria encoding VopC homologs associated with a T3SS; therefore, we predict that these bacteria are also likely to use T3SS-mediated invasion as part of their pathogenesis mechanisms. These findings suggest a new molecular paradigm for . Vibrio pathogenicity and modify our view of the roles of T3SS effectors that are translocated during infection.
AB - Vibrio spp. are associated with infections caused by contaminated food and water. A type III secretion system (T3SS2) is a shared feature of all clinical isolates of . V. parahaemolyticus and some . V. cholerae strains. Despite its being responsible for enterotoxicity, no molecular mechanism has been determined for the T3SS2-dependent pathogenicity. Here, we show that although . Vibrio spp. are typically thought of as extracellular pathogens, the T3SS2 of . Vibrio mediates host cell invasion, vacuole formation, and replication of intracellular bacteria. The catalytically active effector VopC is critical for . Vibrio T3SS2-mediated invasion. There are other marine bacteria encoding VopC homologs associated with a T3SS; therefore, we predict that these bacteria are also likely to use T3SS-mediated invasion as part of their pathogenesis mechanisms. These findings suggest a new molecular paradigm for . Vibrio pathogenicity and modify our view of the roles of T3SS effectors that are translocated during infection.
UR - http://www.scopus.com/inward/record.url?scp=84861685150&partnerID=8YFLogxK
UR - http://www.scopus.com/inward/citedby.url?scp=84861685150&partnerID=8YFLogxK
U2 - 10.1016/j.celrep.2012.04.004
DO - 10.1016/j.celrep.2012.04.004
M3 - Article
C2 - 22787576
AN - SCOPUS:84861685150
SN - 2211-1247
VL - 1
SP - 453
EP - 460
JO - Cell Reports
JF - Cell Reports
IS - 5
ER -