Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression

Bo Pang, Hui Zhang, Jian Wang, Wen Zheng Chen, Shan Hu Li, Qing Guo Shi, Rei Xia Liang, Bang Xiang Xie, Rui Qin Wu, Xiao Long Qian, Lan Yu, Qi Man Li, Cui Fen Huang, Jian Guang Zhou

Research output: Contribution to journalArticle

16 Citations (Scopus)

Abstract

BACKGROUND. Androgen independent prostate cancer (AIPC) is not responsive to androgen ablation therapy. The biomarkers of AIPC are lack. Numerous proteomics studies have focused on finding new markers of AIPC and exploring their possible functions, but little is known about the difference between conditioned medium (CM) from AIPC and androgen dependent prostate cancer (ADPC) cells. METHODS. We performed a proteome analysis of CM from LNCaP, C4-2, and C4-2B cells by a two dimensional electrophoresis based technology. Western blots and immunohistochemical studies were employed to explore the expression pattern of the identified protein in prostate cancer cell lines and clinical specimens, respectively. Then we examined the possible roles and mechanisms of the ubiquitous mitochondrial creatine kinase (uMtCK) in vitro. RESULTS. Besides prostate specific antigen (PSA) and insulin-like growth factor binding protein-2 (IGFBP2), uMtCK was identified in the CM of AIPC cells. uMtCK was up-regulated in AIPC cells and inhuman prostate cancer tissues at WHO grade III. Stably transfected exogenous uMtCK showed a growth promoting effect rather than mock vector in LNCaP cells, with or without bicalutamide in culture medium. Further assays showed that higher degrees of ROS generation and Akt signaling pathway activation in LNCaP-uMtCK than in LNCaP-neo cells. CONCLUSIONS. We showed that uMtCK could be easily detected in CM of LNCaP lineaged AIPC cells. Exogenous uMtCK in LNCaP cells surprisingly contributed to overproduction of ROS, activation of Akt signaling pathway and more aggressive phenotypes including androgen independence development.

Original languageEnglish (US)
Pages (from-to)1176-1187
Number of pages12
JournalProstate
Volume69
Issue number11
DOIs
StatePublished - Aug 1 2009
Externally publishedYes

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Mitochondrial Form Creatine Kinase
Conditioned Culture Medium
Androgens
Prostatic Neoplasms
Cell Line
Insulin-Like Growth Factor Binding Protein 2
Proteome
Prostate-Specific Antigen
Proteomics
Culture Media
Electrophoresis

Keywords

  • Androgen independent
  • Prostate cancer
  • Proteomics
  • Reactive oxygen species
  • Ubiquitous mitochondrial creatine kinase

ASJC Scopus subject areas

  • Oncology
  • Urology

Cite this

Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression. / Pang, Bo; Zhang, Hui; Wang, Jian; Chen, Wen Zheng; Li, Shan Hu; Shi, Qing Guo; Liang, Rei Xia; Xie, Bang Xiang; Wu, Rui Qin; Qian, Xiao Long; Yu, Lan; Li, Qi Man; Huang, Cui Fen; Zhou, Jian Guang.

In: Prostate, Vol. 69, No. 11, 01.08.2009, p. 1176-1187.

Research output: Contribution to journalArticle

Pang, B, Zhang, H, Wang, J, Chen, WZ, Li, SH, Shi, QG, Liang, RX, Xie, BX, Wu, RQ, Qian, XL, Yu, L, Li, QM, Huang, CF & Zhou, JG 2009, 'Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression', Prostate, vol. 69, no. 11, pp. 1176-1187. https://doi.org/10.1002/pros.20969
Pang, Bo ; Zhang, Hui ; Wang, Jian ; Chen, Wen Zheng ; Li, Shan Hu ; Shi, Qing Guo ; Liang, Rei Xia ; Xie, Bang Xiang ; Wu, Rui Qin ; Qian, Xiao Long ; Yu, Lan ; Li, Qi Man ; Huang, Cui Fen ; Zhou, Jian Guang. / Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression. In: Prostate. 2009 ; Vol. 69, No. 11. pp. 1176-1187.
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abstract = "BACKGROUND. Androgen independent prostate cancer (AIPC) is not responsive to androgen ablation therapy. The biomarkers of AIPC are lack. Numerous proteomics studies have focused on finding new markers of AIPC and exploring their possible functions, but little is known about the difference between conditioned medium (CM) from AIPC and androgen dependent prostate cancer (ADPC) cells. METHODS. We performed a proteome analysis of CM from LNCaP, C4-2, and C4-2B cells by a two dimensional electrophoresis based technology. Western blots and immunohistochemical studies were employed to explore the expression pattern of the identified protein in prostate cancer cell lines and clinical specimens, respectively. Then we examined the possible roles and mechanisms of the ubiquitous mitochondrial creatine kinase (uMtCK) in vitro. RESULTS. Besides prostate specific antigen (PSA) and insulin-like growth factor binding protein-2 (IGFBP2), uMtCK was identified in the CM of AIPC cells. uMtCK was up-regulated in AIPC cells and inhuman prostate cancer tissues at WHO grade III. Stably transfected exogenous uMtCK showed a growth promoting effect rather than mock vector in LNCaP cells, with or without bicalutamide in culture medium. Further assays showed that higher degrees of ROS generation and Akt signaling pathway activation in LNCaP-uMtCK than in LNCaP-neo cells. CONCLUSIONS. We showed that uMtCK could be easily detected in CM of LNCaP lineaged AIPC cells. Exogenous uMtCK in LNCaP cells surprisingly contributed to overproduction of ROS, activation of Akt signaling pathway and more aggressive phenotypes including androgen independence development.",
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author = "Bo Pang and Hui Zhang and Jian Wang and Chen, {Wen Zheng} and Li, {Shan Hu} and Shi, {Qing Guo} and Liang, {Rei Xia} and Xie, {Bang Xiang} and Wu, {Rui Qin} and Qian, {Xiao Long} and Lan Yu and Li, {Qi Man} and Huang, {Cui Fen} and Zhou, {Jian Guang}",
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T1 - Ubiquitous mitochondrial creatine kinase is overexpressed in the conditioned medium and the extract of LNCaP lineaged androgen independent cell lines and facilitates prostate cancer progression

AU - Pang, Bo

AU - Zhang, Hui

AU - Wang, Jian

AU - Chen, Wen Zheng

AU - Li, Shan Hu

AU - Shi, Qing Guo

AU - Liang, Rei Xia

AU - Xie, Bang Xiang

AU - Wu, Rui Qin

AU - Qian, Xiao Long

AU - Yu, Lan

AU - Li, Qi Man

AU - Huang, Cui Fen

AU - Zhou, Jian Guang

PY - 2009/8/1

Y1 - 2009/8/1

N2 - BACKGROUND. Androgen independent prostate cancer (AIPC) is not responsive to androgen ablation therapy. The biomarkers of AIPC are lack. Numerous proteomics studies have focused on finding new markers of AIPC and exploring their possible functions, but little is known about the difference between conditioned medium (CM) from AIPC and androgen dependent prostate cancer (ADPC) cells. METHODS. We performed a proteome analysis of CM from LNCaP, C4-2, and C4-2B cells by a two dimensional electrophoresis based technology. Western blots and immunohistochemical studies were employed to explore the expression pattern of the identified protein in prostate cancer cell lines and clinical specimens, respectively. Then we examined the possible roles and mechanisms of the ubiquitous mitochondrial creatine kinase (uMtCK) in vitro. RESULTS. Besides prostate specific antigen (PSA) and insulin-like growth factor binding protein-2 (IGFBP2), uMtCK was identified in the CM of AIPC cells. uMtCK was up-regulated in AIPC cells and inhuman prostate cancer tissues at WHO grade III. Stably transfected exogenous uMtCK showed a growth promoting effect rather than mock vector in LNCaP cells, with or without bicalutamide in culture medium. Further assays showed that higher degrees of ROS generation and Akt signaling pathway activation in LNCaP-uMtCK than in LNCaP-neo cells. CONCLUSIONS. We showed that uMtCK could be easily detected in CM of LNCaP lineaged AIPC cells. Exogenous uMtCK in LNCaP cells surprisingly contributed to overproduction of ROS, activation of Akt signaling pathway and more aggressive phenotypes including androgen independence development.

AB - BACKGROUND. Androgen independent prostate cancer (AIPC) is not responsive to androgen ablation therapy. The biomarkers of AIPC are lack. Numerous proteomics studies have focused on finding new markers of AIPC and exploring their possible functions, but little is known about the difference between conditioned medium (CM) from AIPC and androgen dependent prostate cancer (ADPC) cells. METHODS. We performed a proteome analysis of CM from LNCaP, C4-2, and C4-2B cells by a two dimensional electrophoresis based technology. Western blots and immunohistochemical studies were employed to explore the expression pattern of the identified protein in prostate cancer cell lines and clinical specimens, respectively. Then we examined the possible roles and mechanisms of the ubiquitous mitochondrial creatine kinase (uMtCK) in vitro. RESULTS. Besides prostate specific antigen (PSA) and insulin-like growth factor binding protein-2 (IGFBP2), uMtCK was identified in the CM of AIPC cells. uMtCK was up-regulated in AIPC cells and inhuman prostate cancer tissues at WHO grade III. Stably transfected exogenous uMtCK showed a growth promoting effect rather than mock vector in LNCaP cells, with or without bicalutamide in culture medium. Further assays showed that higher degrees of ROS generation and Akt signaling pathway activation in LNCaP-uMtCK than in LNCaP-neo cells. CONCLUSIONS. We showed that uMtCK could be easily detected in CM of LNCaP lineaged AIPC cells. Exogenous uMtCK in LNCaP cells surprisingly contributed to overproduction of ROS, activation of Akt signaling pathway and more aggressive phenotypes including androgen independence development.

KW - Androgen independent

KW - Prostate cancer

KW - Proteomics

KW - Reactive oxygen species

KW - Ubiquitous mitochondrial creatine kinase

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