Uni-directional ciliary membrane protein trafficking by a cytoplasmic retrograde IFT motor and ciliary ectosome shedding

Muqing Cao, Jue Ning, Carmen I. Hernandez-Lara, Olivier Belzile, Qian Wang, Susan K. Dutcher, Yanjie Liu, William J. Snell

Research output: Contribution to journalArticle

44 Citations (Scopus)

Abstract

The role of the primary cilium in key signaling pathways depends on dynamic regulation of ciliary membrane protein composition, yet we know little about the motors or membrane events that regulate ciliary membrane protein trafficking in existing organelles. Recently, we showed that cilium-generated signaling in Chlamydomonas induced rapid, anterograde IFT-independent, cytoplasmic microtubule-dependent redistribution of the membrane polypeptide, SAG1-C65, from the plasma membrane to the periciliary region and the ciliary membrane. Here, we report that the retrograde IFT motor, cytoplasmic dynein 1b, is required in the cytoplasm for this rapid redistribution. Furthermore, signaling-induced trafficking of SAG1-C65 into cilia is unidirectional and the entire complement of cellular SAG1-C65 is shed during signaling and can be recovered in the form of ciliary ectosomes that retain signal-inducing activity. Thus, during signaling, cells regulate ciliary membrane protein composition through cytoplasmic action of the retrograde IFT motor and shedding of ciliary ectosomes.

Original languageEnglish (US)
JournaleLife
Volume2015
Issue number4
DOIs
StatePublished - Feb 17 2015

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Cell-Derived Microparticles
Cilia
Protein Transport
Membrane Proteins
Membranes
Cytoplasmic Dyneins
Chlamydomonas
Cell signaling
Microtubules
Organelles
Cytoplasm
Cell membranes
Chemical analysis
Cell Membrane
Peptides

ASJC Scopus subject areas

  • Biochemistry, Genetics and Molecular Biology(all)
  • Immunology and Microbiology(all)
  • Medicine(all)
  • Neuroscience(all)

Cite this

Uni-directional ciliary membrane protein trafficking by a cytoplasmic retrograde IFT motor and ciliary ectosome shedding. / Cao, Muqing; Ning, Jue; Hernandez-Lara, Carmen I.; Belzile, Olivier; Wang, Qian; Dutcher, Susan K.; Liu, Yanjie; Snell, William J.

In: eLife, Vol. 2015, No. 4, 17.02.2015.

Research output: Contribution to journalArticle

Cao, Muqing ; Ning, Jue ; Hernandez-Lara, Carmen I. ; Belzile, Olivier ; Wang, Qian ; Dutcher, Susan K. ; Liu, Yanjie ; Snell, William J. / Uni-directional ciliary membrane protein trafficking by a cytoplasmic retrograde IFT motor and ciliary ectosome shedding. In: eLife. 2015 ; Vol. 2015, No. 4.
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AU - Belzile, Olivier

AU - Wang, Qian

AU - Dutcher, Susan K.

AU - Liu, Yanjie

AU - Snell, William J.

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