TY - JOUR
T1 - Upregulation of mir-221 and mir-222 in atypical teratoid/rhabdoid tumors
T2 - Potential therapeutic targets
AU - Sredni, Simone Treiger
AU - Bonaldo, Maria De Fátima
AU - Costa, Fabrício Falconi
AU - Huang, Chiang Ching
AU - Hamm, Christopher Allan
AU - Rajaram, Veena
AU - Tomita, Tadanori
AU - Goldman, Stewart
AU - Bischof, Jared Marshall
AU - Soares, Marcelo Bento
N1 - Funding Information:
Acknowledgments This project was supported by the Dr. Ralph and Marian C. Falk Medical Research Trust, the Rally Foundation for Childhood Cancer Research, and the Maeve McNicholas Memorial Foundation.
PY - 2010
Y1 - 2010
N2 - Purpose: The aim of this study is to search for new therapeutic targets for atypical teratoid-rhabdoid tumors (ATRT). Methods: To achieve this, we compared the expression of 365 microRNAs among ATRT, medulloblastomas, and normal brain. Results: MiR-221 and miR-222 were within the top differentially expressed microRNAs. The deregulated expression of miR221/222 was demonstrated to inhibit the expression of the tumor suppressor and inhibitor of cell cycle p27 Kip1. Here, we demonstrated the negative regulation of p27 Kip1 by miR-221/222 in ATRT using microarray, real-time reverse transcriptase polymerase chain reaction, and immunohistochemistry. Conclusion: As anti-miR therapy was recently proposed as an alternative treatment for cancer, these findings suggest that anti-miR-221/222 therapy might have therapeutic potential in ATRT.
AB - Purpose: The aim of this study is to search for new therapeutic targets for atypical teratoid-rhabdoid tumors (ATRT). Methods: To achieve this, we compared the expression of 365 microRNAs among ATRT, medulloblastomas, and normal brain. Results: MiR-221 and miR-222 were within the top differentially expressed microRNAs. The deregulated expression of miR221/222 was demonstrated to inhibit the expression of the tumor suppressor and inhibitor of cell cycle p27 Kip1. Here, we demonstrated the negative regulation of p27 Kip1 by miR-221/222 in ATRT using microarray, real-time reverse transcriptase polymerase chain reaction, and immunohistochemistry. Conclusion: As anti-miR therapy was recently proposed as an alternative treatment for cancer, these findings suggest that anti-miR-221/222 therapy might have therapeutic potential in ATRT.
KW - Atypical teratoid/rhabdoid tumors
KW - MiR221
KW - MiR222
KW - P27
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UR - http://www.scopus.com/inward/citedby.url?scp=77949268325&partnerID=8YFLogxK
U2 - 10.1007/s00381-009-1028-y
DO - 10.1007/s00381-009-1028-y
M3 - Article
C2 - 20012062
AN - SCOPUS:77949268325
SN - 0256-7040
VL - 26
SP - 279
EP - 283
JO - Child's Nervous System
JF - Child's Nervous System
IS - 3
ER -