Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL

Martha M. Murray, Kurt P. Spindler, Clint Devin, Brian S. Snyder, John Muller, Masaya Takahashi, Percy Ballard, Lillian B. Nanney, David Zurakowski

Research output: Contribution to journalArticle

142 Citations (Scopus)

Abstract

The anterior cruciate ligament (ACL) of the knee fails to heal after primary repair. Here we hypothesize that a beneficial biologic repair response can be induced by placing a collagen-platelet rich plasma (collagen-PRP) material into a central ACL defect. A collagen-PRP scaffold was used to treat a central ACL defect in vivo. In the first experiment, the histologic response in treated and untreated defects was evaluated at 3 (n = 5) and 6 weeks (n = 5). In the second experiment, biomechanical testing of the treated ligaments (n = 8) was performed at 6 weeks and compared with the results of biomechanical testing of untreated defects at the same time-point (n = 6). The percentage filling of the defects in the treated ACLs was significantly higher at both the 3- and 6-week time-points when compared with the untreated contralateral control defects (50 ± 21% vs. 2 ± 2% at 3 weeks, and 43 ± 11% vs. 23 ± 11 at 6 weeks; all values mean ± SEM. Biomechanically, the treated ACL defects had a 40% increase in strength at 6 weeks, which was significantly higher than the 14% increase in strength previously reported for untreated defects (p < 0.02). Placement of a collagen-PRP bridging scaffold in a central ACL defect can stimulate healing of the ACL histologically and biomechanically.

Original languageEnglish (US)
Pages (from-to)820-830
Number of pages11
JournalJournal of Orthopaedic Research
Volume24
Issue number4
DOIs
StatePublished - Apr 2006

Fingerprint

Platelet-Rich Plasma
Anterior Cruciate Ligament
Canidae
Collagen
Ligaments
Knee

Keywords

  • Anterior cruciate ligament
  • Canine
  • Collagen
  • Platelets
  • Wound healing

ASJC Scopus subject areas

  • Orthopedics and Sports Medicine

Cite this

Murray, M. M., Spindler, K. P., Devin, C., Snyder, B. S., Muller, J., Takahashi, M., ... Zurakowski, D. (2006). Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL. Journal of Orthopaedic Research, 24(4), 820-830. https://doi.org/10.1002/jor.20073

Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL. / Murray, Martha M.; Spindler, Kurt P.; Devin, Clint; Snyder, Brian S.; Muller, John; Takahashi, Masaya; Ballard, Percy; Nanney, Lillian B.; Zurakowski, David.

In: Journal of Orthopaedic Research, Vol. 24, No. 4, 04.2006, p. 820-830.

Research output: Contribution to journalArticle

Murray, MM, Spindler, KP, Devin, C, Snyder, BS, Muller, J, Takahashi, M, Ballard, P, Nanney, LB & Zurakowski, D 2006, 'Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL', Journal of Orthopaedic Research, vol. 24, no. 4, pp. 820-830. https://doi.org/10.1002/jor.20073
Murray, Martha M. ; Spindler, Kurt P. ; Devin, Clint ; Snyder, Brian S. ; Muller, John ; Takahashi, Masaya ; Ballard, Percy ; Nanney, Lillian B. ; Zurakowski, David. / Use of a collagen-platelet rich plasma scaffold to stimulate healing of a central defect in the canine ACL. In: Journal of Orthopaedic Research. 2006 ; Vol. 24, No. 4. pp. 820-830.
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