Use of H-2 mutations to quantitate alloreactivity

Alloreactivity is strongest against H-2 antigens which are closest to self

Meade Pimsler, James Forman

Research output: Contribution to journalArticle

5 Citations (Scopus)

Abstract

Lymph-node cells from H-2 allogeneic, intra-H-2 recombinant and H-2 mutant congenic strains were sensitized in limiting dilution cultures to quantitate the cytotoxic T-lymphocyte precursor frequencies (CTL.Pf) against antigens encoded by different regions of the H-2 complex. When four H-2Kb mutants of C57BL/6 (B6) were tested, we observed anti-B6 CTL.Pf that were as high or higher than those of recombinant strains which differ from B6 at the K end of the H-2 complex. Relative to strains completely H-2 allogeneic to B6, the CTL.Pf in H-2bm1, H-2bm3 and H-2bm5 averaged 40-50 percent, and H-2bm8 averaged 140 percent. Recombinant strains B10.A (4R) and B10.D2 (R103), which differ from B6 at the K end of the H-2 complex, averaged 60 percent of the completely H-2 allogeneic value. Since the mutant and wild-type gene products have no serological and minimal structural differences relative to other alleles at H-2K, these results indicate that the CTL.Pf does not increase with increasing H-2 antigenic disparity between any two strains. Rather, the data suggests that the T-cell receptor repertoire recognizes those H-2 molecules or determinants closest to self.

Original languageEnglish (US)
Pages (from-to)111-121
Number of pages11
JournalImmunogenetics
Volume11
Issue number1
DOIs
StatePublished - Dec 1 1980

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H-2 Antigens
Cytotoxic T-Lymphocytes
T-Cell Antigen Receptor
Lymph Nodes
Alleles
Antigens
Mutation
Genes

ASJC Scopus subject areas

  • Immunology
  • Genetics

Cite this

Use of H-2 mutations to quantitate alloreactivity : Alloreactivity is strongest against H-2 antigens which are closest to self. / Pimsler, Meade; Forman, James.

In: Immunogenetics, Vol. 11, No. 1, 01.12.1980, p. 111-121.

Research output: Contribution to journalArticle

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