Use of latex particles for analysis of heterokaryon formation and cell fusion

Maura R. Levine, Rody P. Cox

Research output: Contribution to journalArticle

11 Citations (Scopus)

Abstract

A simple method for the quick and accurate detection of cell fusion utilizing latex particles as cytoplasmic markers was developed and is reported here. The method is particularly useful for demonstrating human skin fibroblast heterokaryons. Ingestion of latex particles did not affect the growth of primary human and established BHK21/C13 hamster fibroblasts. In addition, somatic cell hybridization between hypoxanthine phosphoribosyltransferase-deficient (HPRT-) and thymidine kinase-deficient (TK-) mutants of BHK21/C13 was also unaffected by latex particle ingestion.

Original languageEnglish (US)
Pages (from-to)507-512
Number of pages6
JournalSomatic Cell Genetics
Volume4
Issue number4
DOIs
StatePublished - Jul 1978

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Cell Fusion
Microspheres
Fibroblasts
Eating
Hypoxanthine Phosphoribosyltransferase
Thymidine Kinase
Cricetinae
Skin
Growth

ASJC Scopus subject areas

  • Genetics
  • Medicine(all)

Cite this

Use of latex particles for analysis of heterokaryon formation and cell fusion. / Levine, Maura R.; Cox, Rody P.

In: Somatic Cell Genetics, Vol. 4, No. 4, 07.1978, p. 507-512.

Research output: Contribution to journalArticle

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