Use of latex particles for analysis of heterokaryon formation and cell fusion

Maura R. Levine, Rody P. Cox

Research output: Contribution to journalArticle

11 Scopus citations

Abstract

A simple method for the quick and accurate detection of cell fusion utilizing latex particles as cytoplasmic markers was developed and is reported here. The method is particularly useful for demonstrating human skin fibroblast heterokaryons. Ingestion of latex particles did not affect the growth of primary human and established BHK21/C13 hamster fibroblasts. In addition, somatic cell hybridization between hypoxanthine phosphoribosyltransferase-deficient (HPRT-) and thymidine kinase-deficient (TK-) mutants of BHK21/C13 was also unaffected by latex particle ingestion.

Original languageEnglish (US)
Pages (from-to)507-512
Number of pages6
JournalSomatic Cell Genetics
Volume4
Issue number4
DOIs
StatePublished - Jul 1 1978

ASJC Scopus subject areas

  • Genetics

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