Uterine natural killer cells are targets for a trophoblast cell-specific cytokine, prolactin-like protein A

Heiner Müller, Bing Liu, B. Anne Croy, Judith R. Head, Joan S. Hunt, Guoli Dai, Michael J. Soares

Research output: Contribution to journalArticle

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Abstract

PRL-like protein A (PLP-A) is a member of the PRL family expressed in trophoblast cells coincident with establishment of the chorioallantoic placenta. The purpose of this investigation was to identify targets for PLP-A. Using an alkaline phosphatase-tagging strategy, we show that PLP-A specifically interacts with a population of natural killer (NK) lymphocytes within the mesometrial compartment of decidua from pregnant and pseudopregnant rats. These observations are supported by the codistribution of PLP-A targets with cells expressing the rat NK cell surface marker, gp42, the absence of PLP-A binding in conceptuses from NK cell-deficient tgε26 mice, and the specific interaction of PLP-A with a rat NK cell line, RNK-16. We have further demonstrated that PLP-A effectively suppresses RNK-16 cell cytolytic activities. Our results provide evidence for a new paradigm of embryonic-maternal communication involving a PLP-A signaling pathway between trophoblast cells and uterine NK lymphocytes.

Original languageEnglish (US)
Pages (from-to)2711-2720
Number of pages10
JournalEndocrinology
Volume140
Issue number6
DOIs
StatePublished - 1999

Fingerprint

Staphylococcal Protein A
Trophoblasts
Natural Killer Cells
Prolactin
Cytokines
Lymphocytes
Decidua
Protein Binding
Placenta
Alkaline Phosphatase
Communication
Mothers
Cell Line
Population

ASJC Scopus subject areas

  • Endocrinology
  • Endocrinology, Diabetes and Metabolism

Cite this

Müller, H., Liu, B., Croy, B. A., Head, J. R., Hunt, J. S., Dai, G., & Soares, M. J. (1999). Uterine natural killer cells are targets for a trophoblast cell-specific cytokine, prolactin-like protein A. Endocrinology, 140(6), 2711-2720. https://doi.org/10.1210/en.140.6.2711

Uterine natural killer cells are targets for a trophoblast cell-specific cytokine, prolactin-like protein A. / Müller, Heiner; Liu, Bing; Croy, B. Anne; Head, Judith R.; Hunt, Joan S.; Dai, Guoli; Soares, Michael J.

In: Endocrinology, Vol. 140, No. 6, 1999, p. 2711-2720.

Research output: Contribution to journalArticle

Müller, H, Liu, B, Croy, BA, Head, JR, Hunt, JS, Dai, G & Soares, MJ 1999, 'Uterine natural killer cells are targets for a trophoblast cell-specific cytokine, prolactin-like protein A', Endocrinology, vol. 140, no. 6, pp. 2711-2720. https://doi.org/10.1210/en.140.6.2711
Müller, Heiner ; Liu, Bing ; Croy, B. Anne ; Head, Judith R. ; Hunt, Joan S. ; Dai, Guoli ; Soares, Michael J. / Uterine natural killer cells are targets for a trophoblast cell-specific cytokine, prolactin-like protein A. In: Endocrinology. 1999 ; Vol. 140, No. 6. pp. 2711-2720.
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