UV cross-linking of interacting RNA and protein in cultured cells

Research output: Chapter in Book/Report/Conference proceedingChapter

6 Citations (Scopus)

Abstract

RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

Original languageEnglish (US)
Title of host publicationMethods in Enzymology
PublisherAcademic Press Inc.
Pages53-66
Number of pages14
Volume539
ISBN (Print)9780124201200
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume539
ISSN (Print)00766879
ISSN (Electronic)15577988

Fingerprint

Cultured Cells
Cells
RNA
Immunoprecipitation
Proteins
RNA-Binding Proteins
Irradiation

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

Cite this

Sei, E., & Conrad, N. K. (2014). UV cross-linking of interacting RNA and protein in cultured cells. In Methods in Enzymology (Vol. 539, pp. 53-66). (Methods in Enzymology; Vol. 539). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420120-0.00004-9

UV cross-linking of interacting RNA and protein in cultured cells. / Sei, Emi; Conrad, Nicholas K.

Methods in Enzymology. Vol. 539 Academic Press Inc., 2014. p. 53-66 (Methods in Enzymology; Vol. 539).

Research output: Chapter in Book/Report/Conference proceedingChapter

Sei, E & Conrad, NK 2014, UV cross-linking of interacting RNA and protein in cultured cells. in Methods in Enzymology. vol. 539, Methods in Enzymology, vol. 539, Academic Press Inc., pp. 53-66. https://doi.org/10.1016/B978-0-12-420120-0.00004-9
Sei E, Conrad NK. UV cross-linking of interacting RNA and protein in cultured cells. In Methods in Enzymology. Vol. 539. Academic Press Inc. 2014. p. 53-66. (Methods in Enzymology). https://doi.org/10.1016/B978-0-12-420120-0.00004-9
Sei, Emi ; Conrad, Nicholas K. / UV cross-linking of interacting RNA and protein in cultured cells. Methods in Enzymology. Vol. 539 Academic Press Inc., 2014. pp. 53-66 (Methods in Enzymology).
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