UV cross-linking of interacting RNA and protein in cultured cells

Research output: Chapter in Book/Report/Conference proceedingChapter

7 Scopus citations

Abstract

RNA-protein interactions play indispensable roles in the regulation of cellular functions. Biochemical characterization of these complexes is often done by immunoprecipitation (IP) of RNA-binding proteins (RBPs) followed by identification of co-immunoprecipitated RNAs. This protocol couples ultraviolet (UV) irradiation with IP to determine whether a specific protein interacts directly with a specific RNA in living cells.

Original languageEnglish (US)
Title of host publicationLaboratory Methods in Enzymology
Subtitle of host publicationProtein Part B
PublisherAcademic Press Inc.
Pages53-66
Number of pages14
ISBN (Print)9780124201200
DOIs
StatePublished - 2014

Publication series

NameMethods in Enzymology
Volume539
ISSN (Print)0076-6879
ISSN (Electronic)1557-7988

ASJC Scopus subject areas

  • Biochemistry
  • Molecular Biology

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    Sei, E., & Conrad, N. K. (2014). UV cross-linking of interacting RNA and protein in cultured cells. In Laboratory Methods in Enzymology: Protein Part B (pp. 53-66). (Methods in Enzymology; Vol. 539). Academic Press Inc.. https://doi.org/10.1016/B978-0-12-420120-0.00004-9