Variants of the orexin2/hcrt2 receptor gene identified in patients with excessive daytime sleepiness and patients with Tourette's syndrome comorbidity

Miles D. Thompson, David E. Comings, Rashid Abu-Ghazalah, Yousef Jereseh, Leo Lin, Judy Wade, Takeshi Sakurai, Shigeru Tokita, Tetsuo Yoshida, Hirokazu Tanaka, Masashi Yanagisawa, W. McIntyre Burnham, Harvey Moldofsky

Research output: Contribution to journalArticle

32 Citations (Scopus)

Abstract

The orexin-2/hypocretin-2 (OX2R) receptor gene is mutated in canine narcolepsy and disruption of the prepro-orexin/hypocretin ligand gene results in both an animal model of narcolepsy and sporadic cases of the human disease. This evidence suggests that the structure of the OX2R gene, and its homologue, the OX1R gene, both members of the G protein-coupled receptor (GPCR) family, and the gene encoding the peptide ligands, the prepro-orexin/hypocretin gene, may be variables in the etiology of sleep disorders. We report a single stranded conformational polymorphism (SSCP) analysis of the coding regions of these genes in idiopathic sleep disorder patients diagnosed with excessive daytime sleepiness (EDS) (n = 28), narcolepsy (n = 28), Tourette's syndrome/chronic vocal or motor tic disorder (n = 70), and control subjects (n = 110). Two EDS patients showed a Pro11Thr change. One Tourette's syndrome patient was found to have a Pro10Ser alteration. The Pro10Ser and Pro11Thr variants were not found in non-disease populations. Analysis of the ability of the mutant receptors to mobilize calcium compared to the wild-type receptor in response to orexin agonists indicated that they resulted in decreased potency at high (ηM) concentrations of orexin ligands. Further work is warranted to study the variability of the orexin/hypocretin system in a variety of disorders characterized by EDS.

Original languageEnglish (US)
Pages (from-to)69-75
Number of pages7
JournalAmerican Journal of Medical Genetics - Neuropsychiatric Genetics
Volume129 B
Issue number1
StatePublished - Aug 15 2004

Fingerprint

Tourette Syndrome
Comorbidity
Narcolepsy
Genes
Ligands
Orexin Receptors
Tic Disorders
Single-Stranded Conformational Polymorphism
Aptitude
G-Protein-Coupled Receptors
Canidae
Animal Models
Calcium
Peptides
Orexins
Population

Keywords

  • Narcolepsy
  • Pharmacogenetics
  • Polygenic
  • Receptor variant
  • Tourette's syndrome

ASJC Scopus subject areas

  • Genetics(clinical)
  • Neuropsychology and Physiological Psychology
  • Neuroscience(all)

Cite this

Variants of the orexin2/hcrt2 receptor gene identified in patients with excessive daytime sleepiness and patients with Tourette's syndrome comorbidity. / Thompson, Miles D.; Comings, David E.; Abu-Ghazalah, Rashid; Jereseh, Yousef; Lin, Leo; Wade, Judy; Sakurai, Takeshi; Tokita, Shigeru; Yoshida, Tetsuo; Tanaka, Hirokazu; Yanagisawa, Masashi; Burnham, W. McIntyre; Moldofsky, Harvey.

In: American Journal of Medical Genetics - Neuropsychiatric Genetics, Vol. 129 B, No. 1, 15.08.2004, p. 69-75.

Research output: Contribution to journalArticle

Thompson, MD, Comings, DE, Abu-Ghazalah, R, Jereseh, Y, Lin, L, Wade, J, Sakurai, T, Tokita, S, Yoshida, T, Tanaka, H, Yanagisawa, M, Burnham, WM & Moldofsky, H 2004, 'Variants of the orexin2/hcrt2 receptor gene identified in patients with excessive daytime sleepiness and patients with Tourette's syndrome comorbidity', American Journal of Medical Genetics - Neuropsychiatric Genetics, vol. 129 B, no. 1, pp. 69-75.
Thompson, Miles D. ; Comings, David E. ; Abu-Ghazalah, Rashid ; Jereseh, Yousef ; Lin, Leo ; Wade, Judy ; Sakurai, Takeshi ; Tokita, Shigeru ; Yoshida, Tetsuo ; Tanaka, Hirokazu ; Yanagisawa, Masashi ; Burnham, W. McIntyre ; Moldofsky, Harvey. / Variants of the orexin2/hcrt2 receptor gene identified in patients with excessive daytime sleepiness and patients with Tourette's syndrome comorbidity. In: American Journal of Medical Genetics - Neuropsychiatric Genetics. 2004 ; Vol. 129 B, No. 1. pp. 69-75.
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abstract = "The orexin-2/hypocretin-2 (OX2R) receptor gene is mutated in canine narcolepsy and disruption of the prepro-orexin/hypocretin ligand gene results in both an animal model of narcolepsy and sporadic cases of the human disease. This evidence suggests that the structure of the OX2R gene, and its homologue, the OX1R gene, both members of the G protein-coupled receptor (GPCR) family, and the gene encoding the peptide ligands, the prepro-orexin/hypocretin gene, may be variables in the etiology of sleep disorders. We report a single stranded conformational polymorphism (SSCP) analysis of the coding regions of these genes in idiopathic sleep disorder patients diagnosed with excessive daytime sleepiness (EDS) (n = 28), narcolepsy (n = 28), Tourette's syndrome/chronic vocal or motor tic disorder (n = 70), and control subjects (n = 110). Two EDS patients showed a Pro11Thr change. One Tourette's syndrome patient was found to have a Pro10Ser alteration. The Pro10Ser and Pro11Thr variants were not found in non-disease populations. Analysis of the ability of the mutant receptors to mobilize calcium compared to the wild-type receptor in response to orexin agonists indicated that they resulted in decreased potency at high (ηM) concentrations of orexin ligands. Further work is warranted to study the variability of the orexin/hypocretin system in a variety of disorders characterized by EDS.",
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AU - Lin, Leo

AU - Wade, Judy

AU - Sakurai, Takeshi

AU - Tokita, Shigeru

AU - Yoshida, Tetsuo

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N2 - The orexin-2/hypocretin-2 (OX2R) receptor gene is mutated in canine narcolepsy and disruption of the prepro-orexin/hypocretin ligand gene results in both an animal model of narcolepsy and sporadic cases of the human disease. This evidence suggests that the structure of the OX2R gene, and its homologue, the OX1R gene, both members of the G protein-coupled receptor (GPCR) family, and the gene encoding the peptide ligands, the prepro-orexin/hypocretin gene, may be variables in the etiology of sleep disorders. We report a single stranded conformational polymorphism (SSCP) analysis of the coding regions of these genes in idiopathic sleep disorder patients diagnosed with excessive daytime sleepiness (EDS) (n = 28), narcolepsy (n = 28), Tourette's syndrome/chronic vocal or motor tic disorder (n = 70), and control subjects (n = 110). Two EDS patients showed a Pro11Thr change. One Tourette's syndrome patient was found to have a Pro10Ser alteration. The Pro10Ser and Pro11Thr variants were not found in non-disease populations. Analysis of the ability of the mutant receptors to mobilize calcium compared to the wild-type receptor in response to orexin agonists indicated that they resulted in decreased potency at high (ηM) concentrations of orexin ligands. Further work is warranted to study the variability of the orexin/hypocretin system in a variety of disorders characterized by EDS.

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