Vascular development in the vertebrate pancreas

D. Berfin Azizoglu, Diana C. Chong, Alethia Villasenor, Judith Magenheim, David M. Barry, Simon Lee, Leilani Marty-Santos, Stephen Fu, Yuval Dor, Ondine Cleaver

Research output: Contribution to journalArticle

9 Citations (Scopus)

Abstract

The vertebrate pancreas is comprised of a highly branched tubular epithelium, which is intimately associated with an extensive and specialized vasculature. While we know a great deal about basic vascular anatomy of the adult pancreas, as well as islet capillaries, surprisingly little is known about the ontogeny of its blood vessels. Here, we analyze development of the pancreatic vasculature in the mouse embryo. We show that pancreatic epithelial branches intercalate with the fine capillary plexus of the surrounding pancreatic mesenchyme. Endothelial cells (ECs) within this mesenchyme are heterogeneous from the onset of organogenesis. Pancreatic arteries take shape before veins, in a manner analogous to early embryonic vessels. The main central artery forms during mid-gestation, as a result of vessel coalescence and remodeling of a vascular plexus. In addition, we show that vessels in the forming pancreas display a predictable architecture that is dependent on VEGF signaling. Over-expression of VEGF disrupts vascular patterning and arteriovenous differentiation within the developing pancreas. This study constitutes a first-time in-depth cellular and molecular characterization of pancreatic blood vessels, as they coordinately grow along with the pancreatic epithelium.

Original languageEnglish (US)
Pages (from-to)67-78
Number of pages12
JournalDevelopmental Biology
Volume420
Issue number1
DOIs
StatePublished - Dec 1 2016

Fingerprint

Blood Vessels
Vertebrates
Pancreas
Mesoderm
Vascular Endothelial Growth Factor A
Epithelium
Arteries
Organogenesis
Veins
Anatomy
Embryonic Structures
Endothelial Cells
Pregnancy

Keywords

  • Artery
  • Blood vessel
  • Endothelial
  • Epithelium
  • Heterogeneity
  • Pancreas
  • Plexus
  • VEGF
  • Vein

ASJC Scopus subject areas

  • Molecular Biology
  • Developmental Biology
  • Cell Biology

Cite this

Azizoglu, D. B., Chong, D. C., Villasenor, A., Magenheim, J., Barry, D. M., Lee, S., ... Cleaver, O. (2016). Vascular development in the vertebrate pancreas. Developmental Biology, 420(1), 67-78. https://doi.org/10.1016/j.ydbio.2016.10.009

Vascular development in the vertebrate pancreas. / Azizoglu, D. Berfin; Chong, Diana C.; Villasenor, Alethia; Magenheim, Judith; Barry, David M.; Lee, Simon; Marty-Santos, Leilani; Fu, Stephen; Dor, Yuval; Cleaver, Ondine.

In: Developmental Biology, Vol. 420, No. 1, 01.12.2016, p. 67-78.

Research output: Contribution to journalArticle

Azizoglu, DB, Chong, DC, Villasenor, A, Magenheim, J, Barry, DM, Lee, S, Marty-Santos, L, Fu, S, Dor, Y & Cleaver, O 2016, 'Vascular development in the vertebrate pancreas', Developmental Biology, vol. 420, no. 1, pp. 67-78. https://doi.org/10.1016/j.ydbio.2016.10.009
Azizoglu DB, Chong DC, Villasenor A, Magenheim J, Barry DM, Lee S et al. Vascular development in the vertebrate pancreas. Developmental Biology. 2016 Dec 1;420(1):67-78. https://doi.org/10.1016/j.ydbio.2016.10.009
Azizoglu, D. Berfin ; Chong, Diana C. ; Villasenor, Alethia ; Magenheim, Judith ; Barry, David M. ; Lee, Simon ; Marty-Santos, Leilani ; Fu, Stephen ; Dor, Yuval ; Cleaver, Ondine. / Vascular development in the vertebrate pancreas. In: Developmental Biology. 2016 ; Vol. 420, No. 1. pp. 67-78.
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