Visual neurotransmission in Drosophila requires expression of Fic in glial capitate projections

Mokhlasur Rahman, Hyeilin Ham, Xinran Liu, Yoshie Sugiura, Kim Orth, Helmut Krämer

Research output: Contribution to journalArticle

39 Citations (Scopus)

Abstract

Fic domains can catalyze the addition of adenosine monophosphate to target proteins. To date, the function of Fic domain proteins in eukaryotic physiology remains unknown. We generated genetic models of the single Drosophila Fic domain-containing protein, Fic. Flies lacking Fic were viable and fertile, but blind. Photoreceptor cells depolarized normally following light stimulation, but failed to activate postsynaptic neurons, as indicated by the loss of ON transients in electroretinograms, consistent with a neurotransmission defect. Functional rescue of neurotransmission required expression of enzymatically active Fic on capitate projections of glia cells, but not neurons, supporting a role in the recycling of the visual neurotransmitter histamine. Histamine levels were reduced in the lamina of Fic null flies, and dietary histamine partially restored ON transients. These findings establish a previously unknown regulatory mechanism in visual neurotransmission and provide, to the best of our knowledge, the first evidence for a role of glial capitate projections in neurotransmitter recycling.

Original languageEnglish (US)
Pages (from-to)871-875
Number of pages5
JournalNature Neuroscience
Volume15
Issue number6
DOIs
StatePublished - Jun 2012

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Synaptic Transmission
Neuroglia
Histamine
Drosophila
Recycling
Diptera
Neurotransmitter Agents
Neurons
Photoreceptor Cells
Genetic Models
Adenosine Monophosphate
Light
Proteins
Protein Domains

ASJC Scopus subject areas

  • Neuroscience(all)

Cite this

Visual neurotransmission in Drosophila requires expression of Fic in glial capitate projections. / Rahman, Mokhlasur; Ham, Hyeilin; Liu, Xinran; Sugiura, Yoshie; Orth, Kim; Krämer, Helmut.

In: Nature Neuroscience, Vol. 15, No. 6, 06.2012, p. 871-875.

Research output: Contribution to journalArticle

Rahman, Mokhlasur ; Ham, Hyeilin ; Liu, Xinran ; Sugiura, Yoshie ; Orth, Kim ; Krämer, Helmut. / Visual neurotransmission in Drosophila requires expression of Fic in glial capitate projections. In: Nature Neuroscience. 2012 ; Vol. 15, No. 6. pp. 871-875.
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