Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic

Brian A. Davies, Justin D. Topp, Agnel J. Sfeir, David J. Katzmann, Darren S. Carney, Gregory G. Tall, Andrew S. Friedberg, Li Deng, Zhijian Chen, Bruce F. Horazdovsky

Research output: Contribution to journalArticle

53 Citations (Scopus)

Abstract

Rab5 GTPases are key regulators of protein trafficking through the early stages of the endocytic pathway. The yeast Rab5 ortholog Vps21p is activated by its guanine nucleotide exchange factor Vps9p. Here we show that Vps9p binds ubiquitin and that the CUE domain is necessary and sufficient for this interaction. Vps9p ubiquitin binding is required for efficient endocytosis of Ste3p but not for the delivery of the biosynthetic cargo carboxypeptidase Y to the vacuole. In addition, Vps9p is itself monoubiquitylated. Ubiquitylation is dependent on a functional CUE domain and Rsp5p, an E3 ligase that participates in cell surface receptor endocytosis. These findings define a new ubiquitin binding domain and implicate ubiquitin as a modulator of Vps9p function in the endocytic pathway.

Original languageEnglish (US)
Pages (from-to)19826-19833
Number of pages8
JournalJournal of Biological Chemistry
Volume278
Issue number22
DOIs
StatePublished - May 30 2003

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Ubiquitin
Endocytosis
Proteins
Cathepsin A
Guanine Nucleotide Exchange Factors
Ubiquitin-Protein Ligases
Ubiquitination
GTP Phosphohydrolases
Cell Surface Receptors
Protein Transport
Vacuoles
Yeast
Modulators
Yeasts

ASJC Scopus subject areas

  • Biochemistry

Cite this

Davies, B. A., Topp, J. D., Sfeir, A. J., Katzmann, D. J., Carney, D. S., Tall, G. G., ... Horazdovsky, B. F. (2003). Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. Journal of Biological Chemistry, 278(22), 19826-19833. https://doi.org/10.1074/jbc.M301059200

Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. / Davies, Brian A.; Topp, Justin D.; Sfeir, Agnel J.; Katzmann, David J.; Carney, Darren S.; Tall, Gregory G.; Friedberg, Andrew S.; Deng, Li; Chen, Zhijian; Horazdovsky, Bruce F.

In: Journal of Biological Chemistry, Vol. 278, No. 22, 30.05.2003, p. 19826-19833.

Research output: Contribution to journalArticle

Davies, BA, Topp, JD, Sfeir, AJ, Katzmann, DJ, Carney, DS, Tall, GG, Friedberg, AS, Deng, L, Chen, Z & Horazdovsky, BF 2003, 'Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic', Journal of Biological Chemistry, vol. 278, no. 22, pp. 19826-19833. https://doi.org/10.1074/jbc.M301059200
Davies BA, Topp JD, Sfeir AJ, Katzmann DJ, Carney DS, Tall GG et al. Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. Journal of Biological Chemistry. 2003 May 30;278(22):19826-19833. https://doi.org/10.1074/jbc.M301059200
Davies, Brian A. ; Topp, Justin D. ; Sfeir, Agnel J. ; Katzmann, David J. ; Carney, Darren S. ; Tall, Gregory G. ; Friedberg, Andrew S. ; Deng, Li ; Chen, Zhijian ; Horazdovsky, Bruce F. / Vps9p CUE domain ubiquitin binding is required for efficient endocytic protein traffic. In: Journal of Biological Chemistry. 2003 ; Vol. 278, No. 22. pp. 19826-19833.
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