@article{77a36985f8d24cdb851f32d1980cdb0c,
title = "WASP recruitment to the T cell:APC contact site occurs independently of Cdc42 activation",
abstract = "Cdc42 and WASP are critical regulators of actin polymerization whose function during T cell signaling is poorly understood. Using a novel reagent that specifically detects Cdc42-GTP in fixed cells, we found that activated Cdc42 localizes to the T cell:APC contact site in an antigen-dependent manner. TCR signaling alone was sufficient to induce localization of Cdc42-GTP, and functional Lck and Zap-70 kinases were required. WASP also localized to the T cell:APC contact site in an antigen-dependent manner. Surprisingly, WASP localization was independent of the Cdc42 binding domain but required the proline-rich domain. Our results indicate that localized WASP activation requires the integration of multiple signals: WASP is recruited via interaction with SH3 domain-containing proteins and is activated by Cdc42-GTP concentrated at the same site.",
author = "Cannon, {Judy L.} and Labno, {Christine M.} and Gerra Bosco and Abhinav Seth and McGavin, {Mary H K} and Siminovitch, {Katherine A.} and Rosen, {Michael K.} and Burkhardt, {Janis K.}",
note = "Funding Information: We thank Drs. A. Chan, P. Auvinen, M. Way, J. Krinsje-Locker, G. Van Seventer, J. Miller, and D. Straus for constructs, cell lines, and antibodies. We thank members of the “Polarity group” for many useful discussions. DNA sequencing, peptide synthesis, and microscopy were conducted using the University of Chicago Cancer Research Center Core facilities. We thank Shirley Bond for assistance with microscopy and Jill Voss for administrative assistance. This work was supported by R01 AI44835 and a Career Development award from the Schweppe Foundation to J.K.B. M.K.R. was supported by R01 GM56322 and funding from the Howard Hughes Medical Institute. K.A.S. is a Senior Scientist of the Canadian Institutes for Health Research and was supported by a CIHR grant.",
year = "2001",
doi = "10.1016/S1074-7613(01)00178-9",
language = "English (US)",
volume = "15",
pages = "249--259",
journal = "Immunity",
issn = "1074-7613",
publisher = "Cell Press",
number = "2",
}