Whole-body 12C irradiation transiently decreases mouse hippocampal dentate gyrus proliferation and immature neuron number, but does not change new neuron survival rate

Giulia Zanni, Hannah M. Deutsch, Phillip D. Rivera, Hung Ying Shih, Junie A. Leblanc, Wellington Z. Amaral, Melanie J. Lucero, Rachel L. Redfield, Matthew J. Desalle, Ping-chi B Chen, Cody W. Whoolery, Ryan P. Reynolds, Sanghee Yun, Amelia J Eisch

Research output: Contribution to journalArticle

3 Citations (Scopus)

Abstract

High-charge and-energy (HZE) particles comprise space radiation and they pose a challenge to astronauts on deep space missions. While exposure to most HZE particles decreases neurogenesis in the hippocampus—a brain structure important in memory—prior work suggests that 12C does not. However, much about 12C’s influence on neurogenesis remains unknown, including the time course of its impact on neurogenesis. To address this knowledge gap, male mice (9–11 weeks of age) were exposed to whole-body 12C irradiation 100 cGy (IRR; 1000 MeV/n; 8 kEV/µm) or Sham treatment. To birthdate dividing cells, mice received BrdU i.p. 22 h post-irradiation and brains were harvested 2 h (Short-Term) or three months (Long-Term) later for stereological analysis indices of dentate gyrus neurogenesis. For the Short-Term time point, IRR mice had fewer Ki67, BrdU, and doublecortin (DCX) immunoreactive (+) cells versus Sham mice, indicating decreased proliferation (Ki67, BrdU) and immature neurons (DCX). For the Long-Term time point, IRR and Sham mice had similar Ki67+ and DCX+ cell numbers, suggesting restoration of proliferation and immature neurons 3 months post-12C irradiation. IRR mice had fewer surviving BrdU+ cells versus Sham mice, suggesting decreased cell survival, but there was no difference in BrdU+ cell survival rate when compared within treatment and across time point. These data underscore the ability of neurogenesis in the mouse brain to recover from the detrimental effect of 12C exposure.

Original languageEnglish (US)
Article number3078
JournalInternational Journal of Molecular Sciences
Volume19
Issue number10
DOIs
StatePublished - Oct 9 2018

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Parahippocampal Gyrus
Whole-Body Irradiation
Dentate Gyrus
Bromodeoxyuridine
neurons
Neurons
mice
Brain
Irradiation
Neurogenesis
irradiation
Cells
Cosmic Radiation
brain
Restoration
cells
Radiation
Cell Survival
Astronauts
extraterrestrial radiation

Keywords

  • Carbon
  • Galactic cosmic radiation
  • Hippocampus
  • Neurogenesis
  • Space radiation
  • Subgranular zone

ASJC Scopus subject areas

  • Catalysis
  • Molecular Biology
  • Spectroscopy
  • Computer Science Applications
  • Physical and Theoretical Chemistry
  • Organic Chemistry
  • Inorganic Chemistry

Cite this

Whole-body 12C irradiation transiently decreases mouse hippocampal dentate gyrus proliferation and immature neuron number, but does not change new neuron survival rate. / Zanni, Giulia; Deutsch, Hannah M.; Rivera, Phillip D.; Shih, Hung Ying; Leblanc, Junie A.; Amaral, Wellington Z.; Lucero, Melanie J.; Redfield, Rachel L.; Desalle, Matthew J.; Chen, Ping-chi B; Whoolery, Cody W.; Reynolds, Ryan P.; Yun, Sanghee; Eisch, Amelia J.

In: International Journal of Molecular Sciences, Vol. 19, No. 10, 3078, 09.10.2018.

Research output: Contribution to journalArticle

Zanni, G, Deutsch, HM, Rivera, PD, Shih, HY, Leblanc, JA, Amaral, WZ, Lucero, MJ, Redfield, RL, Desalle, MJ, Chen, PB, Whoolery, CW, Reynolds, RP, Yun, S & Eisch, AJ 2018, 'Whole-body 12C irradiation transiently decreases mouse hippocampal dentate gyrus proliferation and immature neuron number, but does not change new neuron survival rate', International Journal of Molecular Sciences, vol. 19, no. 10, 3078. https://doi.org/10.3390/ijms19103078
Zanni, Giulia ; Deutsch, Hannah M. ; Rivera, Phillip D. ; Shih, Hung Ying ; Leblanc, Junie A. ; Amaral, Wellington Z. ; Lucero, Melanie J. ; Redfield, Rachel L. ; Desalle, Matthew J. ; Chen, Ping-chi B ; Whoolery, Cody W. ; Reynolds, Ryan P. ; Yun, Sanghee ; Eisch, Amelia J. / Whole-body 12C irradiation transiently decreases mouse hippocampal dentate gyrus proliferation and immature neuron number, but does not change new neuron survival rate. In: International Journal of Molecular Sciences. 2018 ; Vol. 19, No. 10.
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abstract = "High-charge and-energy (HZE) particles comprise space radiation and they pose a challenge to astronauts on deep space missions. While exposure to most HZE particles decreases neurogenesis in the hippocampus—a brain structure important in memory—prior work suggests that 12C does not. However, much about 12C’s influence on neurogenesis remains unknown, including the time course of its impact on neurogenesis. To address this knowledge gap, male mice (9–11 weeks of age) were exposed to whole-body 12C irradiation 100 cGy (IRR; 1000 MeV/n; 8 kEV/µm) or Sham treatment. To birthdate dividing cells, mice received BrdU i.p. 22 h post-irradiation and brains were harvested 2 h (Short-Term) or three months (Long-Term) later for stereological analysis indices of dentate gyrus neurogenesis. For the Short-Term time point, IRR mice had fewer Ki67, BrdU, and doublecortin (DCX) immunoreactive (+) cells versus Sham mice, indicating decreased proliferation (Ki67, BrdU) and immature neurons (DCX). For the Long-Term time point, IRR and Sham mice had similar Ki67+ and DCX+ cell numbers, suggesting restoration of proliferation and immature neurons 3 months post-12C irradiation. IRR mice had fewer surviving BrdU+ cells versus Sham mice, suggesting decreased cell survival, but there was no difference in BrdU+ cell survival rate when compared within treatment and across time point. These data underscore the ability of neurogenesis in the mouse brain to recover from the detrimental effect of 12C exposure.",
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AU - Leblanc, Junie A.

AU - Amaral, Wellington Z.

AU - Lucero, Melanie J.

AU - Redfield, Rachel L.

AU - Desalle, Matthew J.

AU - Chen, Ping-chi B

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AU - Reynolds, Ryan P.

AU - Yun, Sanghee

AU - Eisch, Amelia J

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