Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis

Courtney M. Karner, Rani Chirumamilla, Shigehisa Aoki, Peter Igarashi, John B. Wallingford, Thomas J. Carroll

Research output: Contribution to journalArticle

225 Citations (Scopus)

Abstract

Although many vertebrate organs, such as kidneys, lungs and liver, are composed of epithelial tubules, little is known of the mechanisms that establish the length or diameter of these tubules. In the kidney, defects in the establishment or maintenance of tubule diameter are associated with one of the most common inherited human disorders, polycystic kidney disease. Here we show that attenuation of Wnt9b signaling during kidney morphogenesis affects the planar cell polarity of the epithelium and leads to tubules with significantly increased diameter. Although previous studies showed that polarized cell divisions maintain the diameter of postnatal kidney tubules, we find that cell divisions are randomly oriented during embryonic development. Our data suggest that diameter is established during early morphogenetic stages by convergent extension processes and maintained by polarized cell divisions. Wnt9b, signaling through the non-canonical Rho/Jnk branch of the Wnt pathway, is necessary for both of these processes.

Original languageEnglish (US)
Pages (from-to)793-799
Number of pages7
JournalNature Genetics
Volume41
Issue number7
DOIs
StatePublished - Jul 2009

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Kidney Tubules
Cell Polarity
Morphogenesis
Cell Division
Kidney
Polycystic Kidney Diseases
Wnt Signaling Pathway
Embryonic Development
Vertebrates
Epithelium
Maintenance
Lung
Liver

ASJC Scopus subject areas

  • Genetics

Cite this

Karner, C. M., Chirumamilla, R., Aoki, S., Igarashi, P., Wallingford, J. B., & Carroll, T. J. (2009). Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. Nature Genetics, 41(7), 793-799. https://doi.org/10.1038/ng.400

Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. / Karner, Courtney M.; Chirumamilla, Rani; Aoki, Shigehisa; Igarashi, Peter; Wallingford, John B.; Carroll, Thomas J.

In: Nature Genetics, Vol. 41, No. 7, 07.2009, p. 793-799.

Research output: Contribution to journalArticle

Karner, CM, Chirumamilla, R, Aoki, S, Igarashi, P, Wallingford, JB & Carroll, TJ 2009, 'Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis', Nature Genetics, vol. 41, no. 7, pp. 793-799. https://doi.org/10.1038/ng.400
Karner CM, Chirumamilla R, Aoki S, Igarashi P, Wallingford JB, Carroll TJ. Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. Nature Genetics. 2009 Jul;41(7):793-799. https://doi.org/10.1038/ng.400
Karner, Courtney M. ; Chirumamilla, Rani ; Aoki, Shigehisa ; Igarashi, Peter ; Wallingford, John B. ; Carroll, Thomas J. / Wnt9b signaling regulates planar cell polarity and kidney tubule morphogenesis. In: Nature Genetics. 2009 ; Vol. 41, No. 7. pp. 793-799.
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